中华沙鳅(♀)与宽体沙鳅(♂)杂交种的胚胎发育

Embryonic Development of Hybrid of Sinibotia superciliaris(♀) and Botia reevesae(♂)

以中华沙鳅为母本,宽体沙鳅为父本,通过人工杂交获得受精卵,运用数码体视显微镜对杂交种的胚胎发育进行观察拍照,并对杂交种胚胎发育各时期特征进行了详细描述。受精卵直径1.41 mm±0.16 mm,吸水膨胀后直径达1.91 mm±0.09 mm,半漂流性。25℃±0.5℃水温条件下,受精卵历时25 h 50 min孵出。胚胎发育过程分为7个阶段、27个发育期。初孵仔鱼全长4.71 mm±0.12 mm。经统计,杂交种受精率46.84%,孵化率72.84%,仔鱼畸形率8.62%,表明这两种沙鳅属间杂交表现出亲和性。

The hybrids of Sinibotia superciliaris （♀ ） and Botia reevesae （ ♂） were obtained by artificial crossing, and the embryonic development of hybrids was observed using a microscope with digital camera. The fertilized floating eggs had a di- ameter of 1.41 mm ±0.16 mm and would expand to 1.91 mm ±0.09 mm after water swelling. The eggs would drift in flow- ing water. The larvae would be hatched after fertilization for 25 h 50 min at water temperature of 25℃± 0.5℃. The embry- onic development could be divided into 7 main periods including 27 developing stages. The total body length of newly hatched larvae was 4.71 mm± 0. 12 mm. After calculation, the fertilization rate and hatching rate of embryo was about 46. 84% and 72.84% , respectively. Only 8.62% of newly hatched larvae were abnormal. The results showed that the gam- etes from S. superciliaris and B. reevesae are compatible.