参附注射液对窒息型新西兰兔复苏后心肌及脑海马组织超微结构的影响

The Study of myocardium and hippocampus ultrastructure with Shenfu injection in the cardiopulmonary resuscitation

目的研究参附注射液对新西兰兔心搏骤停复苏后心肌组织超微结构的影响，评价其对复苏后心肌组织的保护作用。方法采用窒息法制作新西兰兔心肺复苏模型，随机分为参附注射液组（A组）、正常复苏组（B组）、假手术模型组（c组）。夹闭气管后8min（心搏骤停后约3min）开始心肺复苏，自主循环恢复后A组给予静脉参附注射液2．1ml／kg（生理盐水稀释至5m1），B组给予静脉注射注射生理盐水5ml，每隔30min缓慢静脉注射1次，连续3次；c组静脉仅进行插管等手术操作，不进行呼吸心搏骤停处理和心肺复苏操作。观察复苏后三组动物MAP等变化。自主循环恢复后8h处死动物，取心肌组织和脑海马CA区组织，光镜观察心肌和脑组织的变化、电镜观察超微结构改变。结果在HE染色光镜观察下心肌组织和脑组织的A组和B组之间变化的差异不大，但在电镜观察下则各组间显示差异较大，A组的损害相对较少。结论心肺复苏时应用参附注射液可以减轻心肌和脑组织超微结构损害，对复苏后心肌和脑组织有一定的保护作用。

Objective To explore the protective effect of myocardium and hippocampus ultrastructure with Shenfu injection in the cardiopulmonary resuscitation. Methods Sudden cardiac arrest cardiopulmonary resuscitation models were established by adopting the method of clipping on 24 rabbits who were divided into 3 groups, group A which was injected with SF （2.1ml/kg） after successful resuscitated （SF group）, group B with NS （Sml）, and group C with nothing done. The treatment was repeated 3 times in every 30 minutes. Changes in the MAP during the arrest were observed. The changes in myocardium and hippocampus histiocyte and myocardium and hippocampus uhrastructure were observed with microscope on the rabbits who were executed after 8 hours of successful resuscitation. Results No significant difference was found in myocardium and hippocampus histiocyte between group A and B under the coloring microscope. The myocardium and hippocampus ultrastructure of group B reflected worse than group A, and no change was found in group C. Conclusion SF injection is considered being effective in protecting myocardium and hippocampus from sudden cardiac arrest cardiopulmonary resuscitation.