白细胞介素2基因多态性与手足口病EV71脑炎患者的关联性分析

Association of interleukin-2 gene polymorphism with enterovirus 71 encephalitis in patients with hand, foot, and mouth disease

目的评价人类白细胞介素2-330T／G（IL-2—330T／G，rs2069762）基因多态性与肠道病毒EV71脑炎之间的关联。方法从5700例疑似手足LI病（HFMD）患儿中选择115名EV71型感染脑炎患儿和256名无合并症的EV71型HFMD患儿进行关联性分析。用荧光定量PCR进行Ev71型病毒的诊断。用高分辨熔解曲线技术分析和PCR—DNA测序法确定IL-2基因的多态性。结果在EV71型脑炎的患儿组中IL-2—330TT＋TG基因型的频率（93．91％）明显的高于EV71型感染的无合并症HFMD组（74．61％），两者之间存在统计学差异（P=0．000）。IL-2．330T等位基因的频率在EV71型脑炎的患儿组（70．43％）明显的高于EV71型感染的无合并症HFMD组（51．95％），是-个危险『生因素（OR=2．203，95％CI=0．127～0．756，P=0．001）。结论我们的研究结果表明，IL-2．330T等位基因是-个EV71型脑炎风险增加密切相关的因素。

Objective To investigate the association between EV71 encephalitis and IL-2-330 T/G （rs2069762） gene polymorphism. Methods The study was performed in 115 patients with EV71 encephalitis and 256 patients with EV71-related HFMD without complications from 5 700 suspected HFMD cases. EV71 was identified by fluorescent quantitative PCR. Genotyping was determined by high resolution melting assay （HRMA） and PCR-DNA sequencing. Results The patients with EV71 encephalitis had a significantly higher frequency of the IL-2-330 TT＋TG genotypes （93.91%） as compared to the patients with EV71-related HFMD without complications （74.61%, P=0.000）. The frequency of IL-2-330 T alleles was also significantly higher among the patients with EV71 encephalitis （70.43%） as compared to that of the patients with EV71-related HFMD without complications （51.95%, OR=2.203, 95%CI=0.127-0.756, P=0.001）. Conclusion These findings suggested that the IL-2-330 T allele could be significantly associated with risk of encephalitis in patients with EV71-related HFMD.