慢性阻塞性肺病患者外周血中CD34^+细胞数量变化

Change of EPCs in perpherail blood of COPD patients

目的:观察中、重度慢性阻塞性肺病(COPD)患者外周血中CD34+细胞数量变化。方法:按入选标准收集25例中、重度COPD患者外周血,应用密度梯度离心法分离单个核细胞,采用CD34+正分选试剂盒进行磁珠纯化,用流式细胞分析仪分析其纯度,同时对入选患者行常规心电图、肺功能、心动超声及血气检查。同期16例性别、年龄无差异志愿者作为对照组,入选标准:年龄<70岁;肺功能正常;没有临床症状;无吸烟史。结果:经过分离纯化可得到93%表达CD34+细胞。COPD患者组与对照组外周血中的CD34+细胞数分别是(19.99±11.11)×104、(32.75±18.25)×104,患者组CD34+细胞数明显少于对照组(P<0.05),CD34+细胞数与患者病程呈负相关(r=-0.689,P<0.05)。结论 :COPD患者外周血中CD34+细胞数降低,与患者病程呈负相关。

Objective. To observe the change of EPCs in perpherail blood of chronic obstructive pulmonary disease （COPD） patients. Methods. Based on the selection standard, peripheral blood was col- lected from media and severe COPD patients. Peripheral blood mononuclear cells （PBMCs） were obtained by standard Ficoll gradient centrifugation, and purified by CD34^＋ positive selection cocktail. All subjects received a complete clinical assessment including pulmonary function tests, a 12-lead ECG and blood gas analysis. The selection standard of control group was as follows, age〈70 years old, normal pulmonary function, no clinical symptoms or smoking history. Results： 93% CD34^＋ cells was obtained after isolation and purification. The count of CD34^＋ cells was （19.99±11.11） ×10^4 and （32.75±18.25）×10^4in patients group and control group, respectively. The count of patients group was significantly lower （P〈0.05）. There were negative correlation between CD34^＋ cell counts and course of COPD（r=-0. 689 ,P〈0.05）. Conclusions： The count of CD34^＋ is decreased, and is negatively correlated with course of COPD.