修复基因XRCC1和XPD多态性及交互作用对铅毒性易感性的影响

Influence on susceptibility to lead toxicity in repair genes XRCC1 and XPDwith polymorphisms and interaction

目的研究XRCC1、XPD基因多态性及交互作用与铅毒性易感性的关系。方法采集326名某蓄电池企业铅作业工人静脉血和晨尿,检测血铅、血锌原卟啉(ZPP)和尿铅,用PCR-RFLP检测XRCC1、XPD的基因多态性,分析不同基因型及交互作用对铅毒性易感性的影响。结果 XRCC1-194CT+TT基因组平均血铅值较XRCC1-194CC组高;病例组XRCC1-194CT+TT基因型比例大于对照组,慢性铅中毒发生率风险较XRCC1-194CC组高。结论在相同职业铅暴露环境下,XRCC1-194CT+TT基因型工人对铅毒性易感;XPD Asp312Asn和Lys751Gln多态性与铅毒性易感性无关;XRCC1Arg194Trp、XPD Asp312Asn和Lys751Gln基因对铅毒性的影响不存在交互作用。

Objective To explore the influence on susceptibility to lead toxicity in repair genes XRCC1 and XPD with polymorphisms and interaction. Methods Totally 326 blood and urine samples of leadexposed workers occupationally were collected from a storage battery factory.Lead and ZPP level in blood and lead level in urine were detected.The genotype polymorphisms of the XRCC1, XPD gene were detected by PCRRFLP method.The influence on susceptibility to lead toxicity of the gene polymorphisms and interaction were analyzed. Results The blood lead level of XRCC1194CT＋TT genotype was higher than that of XRCC1194CC genotype.The proportion of XRCC1194CT＋TT genotype in case groups was higher than that of XRCC1194CC genotype, and the risk of chronic lead poisoning was higher than that of XRCC1194CC genotype. Conclusion With the same occupationally lead exposure workplace, the workers with XRCC1194CT＋TT genotype shows higher risk of chronic lead poisoning.The XPDAsp312Asn and XPDLys751Gln polymorphisms is not one factor of susceptible to lead toxicity.There is no obvious interaction for lead poisoning in XRCC1 Arg194Trp, XPD Asp312Asn and XPD Lys751Gln genotype.