头穴丛刺结合丰富环境刺激对缺氧缺血性脑损伤新生大鼠突触超微结构和EGFmRNA表达的影响

Influence of Scalp Cluster Acupuncture Combined with Enriched Environmental Stimulation on Synaptic Ultrastructure and EGFmRNA in Newborn Rats with Hypoxic-ischemic Brain Damage

目的:观察头穴丛刺结合丰富环境刺激对新生大鼠缺氧缺血性脑损伤后突触超微结构和EGFmRNA表达的影响。方法:采用Rice法建立HIBD动物模型。随机分为造模组(A)、头穴丛刺组(B)、丰富环境刺激组(C)、头穴丛刺结合丰富环境刺激组(D)。选取7 d、14 d、21 d,应用透射电镜和原位杂交技术观察头穴丛刺结合丰富环境刺激对缺氧缺血性脑损伤新生大鼠突触超微结构和EGFmRNA表达的影响。结果:头穴丛刺结合丰富环境刺激组突触结构好,突触小泡数量较多,神经元细胞线粒体水肿明显减轻;14 d、21 d头穴丛刺结合丰富环境刺激组EGFmRNA表达明显高于相同时间段头穴丛刺组和丰富环境刺激组(P<0.01)。结论:头穴丛刺结合丰富环境刺激能够明显减轻缺血缺氧所致的突触超微结构损伤和增加EGFmRNA的表达。

Objective：To investigate effect of scalp cluster acupuncture combined with enriched environmental stimulation on synaptic ultrastructure and EGFmRNA in newborn rats with hypoxic -ischemic brain damage . Methods：HIBD model was established by Rice method .Rats were randomly divided into four groups：A-model group ,B-scalp cluster acupuncture group ,C-enriched environmental stimulation group and D -scalp cluster acupuncture combined with enriched environmental stimulation group .After 7 days,14 days and 21 days,ob-serve the effect of scalp cluster acupuncture combined with enriched environmental stimulation on synaptic ultra -structure and EGFmRNA by using addr .TEM and in situ hybridization （ ISH） .Results：D group was fine of syn-aptic ultrastructure and of the large number of synaptic vesicles and neuron mitochondria swelling significantly reduced.At the same time, in D group at the 14th day and the 21st day,the expression of EGFmRNA was obvi-ously higher than the same period in B group and C group （P〈0.01）.Conclusion：Scalp cluster acupuncture combined with enriched environmental stimulation could significantly reduce the damage of synaptic ultrastruc -ture by HI and increase the expression of EGFmRNA .