期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

肝细胞核因子-1α对小鼠Ⅰ型葡萄糖载体表达的影响

Effect of hepatocyte nuclear factor 1α on expression of mouse glucose transporterⅠin CV-1 cells
下载PDF
导出
摘要 [目的]探讨在CV-1细胞中肝细胞核因子-1α(HNF-1α)对小鼠Ⅰ型葡萄糖载体(mGLUT1)表达的影响.[方法]利用DNA克隆技术,将HNF-1αcDNA序列重组到表达载体pSV-sport上,以HNF-1α转染CV-1细胞,应用Northern blot方法测定HNF-1α对mGLUT1 mRNA表达的影响.[结果]HNF-1α可提高mGLUT1 mRNA表达水平及稳定性.[结论]HNF-1α可增强mGLUT1的表达,可能通过调节GLUT1的表达参与胰岛素对血糖的调节过程. OBJECTIVE To study the effect of hepatocyte nuclear factor lu （HNF-1α） on expression of mouse glucose transporter I （mGLUT1） in CV-1 cells. METHODS Using DNA cloning technology, HNF-1α cDNA sequence recombined into pSV-sport expression vector, and the recombinant HNF-1α was transfected into CV-1 cells, and the effect of HNF-1α on expression of mGLUT1 mRNA was detected by Northern blot. RESULTS HNF-1α can increase the expression and stability of mGLUT1 mRNA. CONCLUSION HNF-1α increases expression of mGLUT1, and may participate in regulating process of insulin for blood glucose by regulating the expression of mGLUT1.
作者 李天洙 张晓庆 崔敬兰
机构地区 延边大学医学院生物化学与分子生物学教研室
出处 《延边大学医学学报》 CAS 2013年第4期260-262,共3页 Journal of Medical Science Yanbian University
基金 吉林省教育厅科学技术研究项目
关键词 肝细胞核因子 葡萄糖转运蛋白质类 易化性 小鼠 hepatocyte nuclear factor glucose transport proteins, facilitative mice
分类号 R363 [医药卫生—病理学]
  • 相关文献

参考文献11

  • 1McPherson CE, Shelton EY, Friedman DS, et at: An active tissue-specific enhancer and bound transcription factors existing in a precisely positioned nucleosomal array[J]. Cell, 1993,75 : 387-398.
  • 2Lai E, Prezioso VR, Tao WF, et al: Hepatocyte nu- clear factor 3 belong to a gene family in mammals that is homologous to the Drosophila homeotic gene forkhead FJ]. Genes Dev, 1991,5:416-427.
  • 3Song YH, Ray K, Liebhaber SA, et al: Vitamin D-binding protein gene transcription is regulated by therelative abandance of hepatocyte nuclear factors la and 1 [J]. J Biol Chem, 1998,264 : 28408-28418.
  • 4Miura N, Tanaka K. Analysis of the rat hepatocyte nu-clear factor(HNF) 1 gene promoter: synergistic activa- tion by HNF4 and HNF1 proteins [J]. Nucleic Acids Res, 1993,21 : 3731-3736.
  • 5Wang H, Maechler P, Hagenfeldt KA, et at: Domi- nant-negative suppression of HNFla function results in defective insulin gene transcription and impaired me- tabolisom-secretion coupling in a pancreatic -cell line PJ]. EMBO J, 1998,17 : 6701-6713.
  • 6Chen QI, Phillip H, Pekala. The influence of mRNA stability on glucose transporter (GLUT1) gene expres- sion[J]. BBRC, 1999,263 : 265-269.
  • 7Yoko O, Masahiro S, Toshiya Y. Involement of nuclear transcription factor Spl in regulating glucose transport- er -1 gene expression during rat trophoblast differentia- tion[J]. BBRC , 2001, 288 : 940-948 .
  • 8Flores-Riveros JR, McClenithan JC, Ezaki O, et al: Insulin down-regulates expression of the insulin-re- sponsive glucose transporter (GLUT4) gene: effects on transcription and mRNA turnover[J]. Proc Natl Acad Sci USA ,1993,90:512-516.
  • 9Pai J, Guryev O, Brown MS, et al: Differential stim- ulation of cholesterol and unsaturated fatty acid biosyn- thesis in cells expressing individual nuclear sterol regu- latory element-binding proteins E J ]. J Biol Chem , 1998,273 : 26138-26148.
  • 10Pardridge WM, Triguero D, Farrell CR. Down regula- tion of blood-brain glucose transporter in experimental diabetes[J]. Diabetes, 1990,39 : 1040-1044.
延边大学医学学报
2013年 第4期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部