血小板源性生长因子(PDGF)、血清反应因子(SRF)和波形蛋白在人脑胶质瘤中表达的临床意义

Expression and clinical significance of platelet-derived growth factor(PDGF),serum response factor(SRF)and vimentin in human gliomas

目的研究血小板源性生长因子(platelet-derived growth factor,PDGF)、血清反应因子(serum response factor,SRF)和波形蛋白(vimentin)在人脑胶质瘤中的表达及其临床意义。方法采用免疫组化染色法检测13例正常脑组织和70例人脑胶质瘤组织中PDGF-B、SRF和波形蛋白的表达并分析其临床意义。结果 PDGF-B、SRF和波形蛋白在人脑胶质瘤组织中高表达,阳性表达率分别为67.14%、48.57%和81.43%,差异有统计学意义(P<0.05);三者在脑胶质瘤中的表达与患者性别、年龄、肿瘤发生部位、肿瘤大小无关(P>0.05)。随着胶质瘤恶性程度增加,三者表达逐渐上调,其中PDGF-B、波形蛋白在脑胶质瘤中的表达与肿瘤分级相关;相关性分析显示PDGFB与SRF、波形蛋白在脑胶质瘤中的表达有相关性(P<0.05)。结论PDGF-B、SRF和波形蛋白在脑胶质瘤的发生、发展中可能起到重要的调节作用,与肿瘤恶性程度具有一定的相关性。

Objective To explore the expression and clinical significance of platelet-derived growth factor （PDGF）, serum response factor （SRF） and vimentin in human gliomas. Methods Seventy expressions of PDGF-B, SRF and vimentin protein form 70 cases of glioma tissue and 13 cases of normal brain tissue were measured by immunohistochemistry stain. The correlations were analyzed between the changes of PDGF,SRF and vimentin protein expressions and clinical biological behavior of glioma,including sex,age,tumor size and location,pathological grade. Results Immuohistochemical staining revealed that the positive rate of PDGF-B, SRF and vimentin were 67. 14%, 48. 57% and 81.43% respectively in gliorna tissues, which were higher than those in normal brain tissues （P〈 0.05）. The expressions of PDGF-B, SRF and vimentin were not correlated with sex, age, tumor size and location （P〉0.05） and increased by malignancy of glioma. The positive rate of PDGF-B and vimentin were significantly higher in high-grade gliomas （m-IV） than those in low-grade gliomas （ I -H ） （P〈 0.05）. There is a positive correlation between PDGF-B and SRF or vimentin through spearman＇s and Z2 test （r= 0. 246,P= 0. 034;r= 0. 347,P = 0. 002）. Conclusions The up-regulation of PDGF-B,SRF and vimentin may play important roles in the occurrence and development of glioma.