顽固性便秘病人结肠黏膜菌群的变化特征

Alteration of colonic mucosa-associated microbiota in patients with refractory constipation

目的:阐释顽固性便秘病人结肠黏膜菌群的变化特征。方法:本研究分为顽固性便秘组(n=10)和对照组(n=6)。通过结肠镜活检获取乙状结肠黏膜标本,提取DNA,PCR扩增后行变性梯度凝胶电泳,应用分子生物学分析软件对菌群结构的相似性、主成分和多样性进行分析。结果:顽固性便秘病人结肠黏膜菌群有明显改变,与对照组比,顽固性便秘病人结肠黏膜菌群物种丰富度和香农多样性指数均显著降低(P<0.01)。结论:顽固性便秘病人结肠黏膜菌群紊乱,表现为结肠黏膜菌群多样性减少。

Objective： Previous studies suggested that changes existed in cies in the stool of chronic functional constipation, but the composition of colonic erobiota in refractory constipation （RC） is unknown. Our aim was to investigate colonic mucosa-associated microbiota in RC. Methods： Ten RC patients and 6 selected bacterial spe- mucosa-associated mi- the characterization of controls were included in this study. Mucosal tissues from sigmoid colon were obtained by biopsy through colonoscopy. Mucosa- associated microbiota was analyzed by Polymerase Chain Reaction-Denaturing Gradient Gel Electrophore- sis （PCR-DGGE）. Results ： Colonic mucosa-associated microbiota composition of RC patients was dif- ferent from the controls. Band profiles revealed that the number of predominant bacteria was significantly different in RC. A significant decrease in bacterial species and biodiversity in RC was suggested 0.01 ）. Conclusion： The abnormalities of colonic mucosa-associated microbiota in RC are characterized by a decrease in biodiversity. （P〈 mainly