血管内皮生长因子C及受体与胃癌淋巴转移关系的研究

Relationship of vascular endothelial growth factor C and its receptor-3 with lymphatic metastasis of gastric cancer

目的探讨血管内皮生长因子-C(VEGF-C)及其受体-3(VEGFR-3)在胃癌中的表达及其与淋巴结转移的关系。方法检测延安大学附属医院2012年1月-2013年1月收治的78例原发性胃癌患者胃黏膜VEGF-C和VEGFR-3蛋白的表达,并与20例胃癌患者经病理证实为正常胃黏膜的远切端组织进行比较,分析胃癌淋巴结转移情况。结果 VEGF-C在正常胃黏膜组织中呈阴性表达,在胃癌组织中定位于癌细胞胞浆中,呈弥散性分布,78例胃癌组织中,60例表达阳性,阳性率为76.92%,胃癌组织与正常胃黏膜组织VEGF-C阳性率差异有统计学意义(P<0.01);VEGF-C的表达与组织学分级、浸润深度、有无淋巴转移及TNM分期密切相关。VEGFR-3在正常胃黏膜细胞中着色均匀,主要分布于细胞胞浆内,阳性表达率为10.00%;在胃癌组织中的表达异质明显,呈灶状或弥散性分布,阳性率为66.67%,差异有统计学意义(P<0.01);VEGFR-3表达与组织学分级、有无淋巴转移及TNM分期密切相关。VEGF-C与VEGFR-3表达呈正相关。结论 VEGF-C及其受体VEGFR-3在胃癌的发生、发展及淋巴转移中发挥着重要作用。

Objective To investigate the relationship of vascular endothelial growth factor C （VEGF-C） and its re- ceptor-3 （VEGFR-3） with lymphatic metastasis of gastric cancer. Methods Seventy-eight cases of primary gastric cancer patients in our hospital from Jan. 2012 to Jan. 2013 were collected to detect expression of VEGF-C and VEGFR- 3 proteins. Compared with 20 cases of normal gastric tissue distal cut end of pathologically confirmed gastric cancer pa- tients, lymph node metastasis was analyzed. Results Expression of VEGF-C was negative in normal gastric mucosa. VEGF-C located in the cytoplasm of cancer ceils in gastric cancer tissues and showed a diffuse distribution. In 78 cases of gastric cancer, expression of VEGF-C was positive in 60 cases, the positive rate was 76.92% , there was statistically significant in VEGF-C positive rate between normal gastric mucosa group and gastric cancer group （P 〈 0.01 ）. VEGF- C expression was related with histological grade, depth of invasion, lymph node metastasis and TNM staging. VEGFR-3 in normal gastric mucosa cells coloured uniformity, mainly distributed in the cytoplasm, the positive expression rate was 10.00%; expression of VEGFR-3 in gastric cancer tissues was spotty or diffuse distribution, the positive rate was 66.67%, there was statistically significant in VEGF-C positive rate between normal gastric mucosa group and gastric cancer group （P 〈 0.01 ） ; VEGFR-3 expression was related with histological grade, lymph node metastasis and TNM staging. VEGF-C and VEGFR-3 expression was positively correlated. Conclusion VEGF-C and VEGFR-3 play impor- tant roles in occurrence, development and lymphatic metastasis of gastric cancer.