生存素在慢性肝病患者外周血中的表达

Expression of serum survivin in patients with chronic hepatopathy

目的评价慢性肝病患者外周血生存素检测对慢性肝病的诊断价值。方法将研究对象分为原发性肝癌组、乙肝肝硬化组、慢性乙型肝炎组和正常对照组。各组采集空腹外周静脉血3 ml,通过双抗体夹心酶联免疫吸附测定法检测生存素浓度。结果原发性肝癌组、乙肝肝硬化组、慢性乙型肝炎组和正常对照组外周血生存素浓度分别为(83.70±14.45)ng/L、(70.58±9.78)ng/L、(67.90±9.14)ng/L和(65.07±7.58)ng/L。慢性乙型肝炎组与正常对照组比较,差异无统计学意义(P>0.05),乙肝肝硬化组与正常对照组比较,差异有统计学意义(P<0.05),原发性肝癌组外周血生存素浓度明显高于乙肝肝硬化组、慢性乙型肝炎组和正常对照组,差异有显著统计学意义(P<0.01)。根据试验结果绘制受试者工作特征(ROC)曲线,原发性肝癌组ROC曲线下面积为0.82。结论外周血生存素是一种有诊断价值的评价慢性肝病的血清学指标,尤其对于原发性肝癌的诊断具有一定的价值。

Objective To evaluate the diagnostic value of serum survivin in patients with chronic hepatopathy. Methods Enzyme linked immunosorbent assay was used to detect the serum survivin in normal adults, patients with chronic hepatitis B, patients with hepatitis B associated liver cirrhosis and patients with primary liver cancer （PLC）. Results The level of survivin in patients with PLC （83.70 ± 14.45） ng/L was significantly higher than that in patients with hepatitis B associated liver cirrhosis （70.58 ± 9.78） ng/L, chronic hepatitis B （67.90 ± 9.14） ng/L, and normal adults （65.07 ± 7.58 ） ng/L （P 〈 O. 01 ）. The difference of levels of survivin in patients with hepatitis B associated liv- er cirrhosis and normal adults was significant （P 〈 O. 05 ）. But the difference of the serum survivin levels in patients with hepatitis B associated liver cirrhosis and chronic hepatitis B was not significant. ROC curve was built according to the test value. The area under the curve was 0.82 （P 〈 0.01 ）. Conclusion Serum survivin may be useful for diagnosis of hepatitis B associated liver cirrhosis and it can be an important diagnosis marker of PLC.