IL-33在β-淀粉样蛋白刺激视网膜色素上皮细胞中的表达及作用研究

The expression and inflammatory role of IL-33 in amyloid-β stimulated retinal pigment epithelium cells

为探讨年龄相关性黄斑变性的免疫炎症反应机制,采用real-time PCR及ELISA检测β-淀粉样蛋白1-40(Aβ_((1-40)))刺激RPE细胞后IL-33 mRNA及蛋白水平表达,并采用ELISA检测IL-33刺激RPE细胞后炎症因子表达情况及调控产生炎症因子的信号通路。结果发现,Aβ_((1-40))刺激人视网膜色素上皮细胞后能够诱导IL-33因子转录及蛋白水平表达上调,且在人视网膜色素上皮细胞中,IL-33因子分别通过p38 MAPK、ERK1/2信号通路调控促炎症因子IL-6、IL-8的表达。据此说明,IL-33通过调控人视网膜色素上皮细胞产生促炎症因子IL-6、IL-8参与年龄相关性黄斑变性的发生发展。

To investigate the immune inflammatory response mechanism of age-related macular degeneration, IL-33 mRNA and protein expression levels were detected by real-time PCR and ELISA after retinal pigment epithelium （RPE） cells were treated with Aβ1-40. Proinflammatory factors expression and signaling pathways were examined by ELISA when RPE cells were stimu- lated with IL-33. The results showed that IL-33 expression by RPE cells was increased after Aβ1-40 stimulation and IL-33 regulated IL-6 and IL-8 secretion by activating p38 MAPK and ERK1/2 signaling pathways, respectively. Taken together, IL33 takes part in the development of age-related macular degeneration by regulating RPE cells to produce proinflammatory factor IL- 6 and IL-8..