摘要
目的了解胰腺导管腺癌（PDAC）中白血病抑制因子（LIF）表达相关性及其临床病理学意义，并探讨白血病抑制因子对胰腺癌细胞生物学行为的影响。方法应用免疫组织化学方法检测53例配对的PDAC及癌旁组织石蜡标本LIF蛋白表达水平，并分析LIF表达与患者临床病理学各项指标的关系，再以Western印迹法检测14对配对冷冻保存的新鲜PDAC和癌旁组织中LIF表达水平，然后运用Western印迹和聚合酶链反应（PCR）检测6株胰腺癌细胞系（AsPC-1、BxPC-3、PANC-1、SW-1990、Capan-2与Miapaca．2）中自血病抑制因子（LIF）蛋白和LIF—RmRNA表达水平，再运用MTr、Transwell实验检测LIF对胰腺癌细胞系增殖、侵袭及迁移的影响。结果在53例胰腺癌组织中LIF的阳性表达率为66．O％（35／53），在癌旁非肿瘤性胰腺导管中阳性表达率为35．8％（19／53）；LIF在癌组织中表达明显高于配对的癌旁组织（66．0％与35．8％；t：3．031，P=0．004）；LIF表达与肿瘤TNM分期（）（2=3．635，P=0．057）、浸润深度（x。=3．726，P：0．054）差异无统计学意义，但有明显的趋势效应。LIF在14对PDAC中表达水平同样高于癌旁组织（t：5．283，P〈0．01）。免疫印迹和PCR显示：LIF因子在Capan-2中的表达水平相对低于其他5株细胞系，而其受体mRNA在Capan-2细胞中的表达水平相对高于其余5株细胞系。LIF因子能够促进Capan-2细胞的增殖（P〈0．05）。并且能够增加Capan-2细胞的迁移及侵袭能力（P〈0．05）。结论LIF在胰腺癌高表达可能参与胰腺癌的发生进展，且在部分胰腺癌细胞系中，LIF能够促进胰腺癌细胞增殖、侵袭和迁移。
Objective To explore the expression of leukemia inhibitory factor （LIF） and its clinicopathological significance in haman pancreatic ductal adenocarcinoma （PDAC） and examine its regulatory role of biological behaviors of pancreatic cancer cells. Methods The expression of LIF protein in 53 paired paraffin-embedded PDAC specimens and adjacent non-cancerous pancreatic tissues were detected by immunohistoehemistry. The relationship between their expressions and clinicopathological characteristics was analyzed. Western bolt was used to examine the expression of LIF level in 14 paired fresh PDAC specimens and adjacent non-cancerous pancreatic tissues. Furthermore, Western blot and polymerase chain reaction （PCR） were used to detect the LIF-R mRNA and protein level of LIF in 6 pancreatic cancer cell lines （AsPC-1, BxPC-3, PANC-1, SW-1990,Capan-2 and Miapaca-2）. And the assays of MTF, invasion and migration were used to detect the effects of LIF in regulating cell proliferation, invasion and migration in pancreatic cancer cell lines. Results The expression of LIF increased in 53 cases of PDAC versus paired normal tissues （66. 0% vs 35.8% ; t = 3. 031 ,P = 0. 004）. And its positive association with tumor TNM stage （X^2 = 3. 635,P = 0.057） and invasion depth （X^2 = 3. 726, P = 0.054） had no statistical significance. Univafiate analysis revealed that LIF may be a correlative adverse prognostic indicator for patients with PDAC （X^2 =3. 233 ,P =0. 072）. The expression of LIF was much higher in 14 cases of PDAC than that in adjacent normal pancreatic tissues （t = 5. 283 ,P 〈 0.01 ）. The protein level of LIF was lower in Capan-2 cells than that in other 5 pancreatic cancer cell lines while mRNA level of LIF receptor was higher than that in other 5 cell lines. Also LIF could promote the proliferation, migration and invasion of Capan-2 cell （ P 〈 0. 05 ） . Conclusion An over-expression of LIF may contribute to the development and progression of PDAC. LIF can promote the proliferation, migration and invasion in some pancreatic cancer cells.
出处
《中华医学杂志》
CAS
CSCD
北大核心
2014年第2期90-95,共6页
National Medical Journal of China
基金
辽宁省自然科学基金（20092131）
