摘要
目的分析2009--2011年广州地区甲型HINl流感病毒对奥司他韦的耐药情况。方法选取2009年7月至2011年4月从广州地区分离的甲型HlNl流感病毒192株，通过对病毒的血凝素和神经氨酸酶基因进行序列测定，并利用数据库和软件进行生物信息学分析；测定病毒对奥司他韦的敏感度，分析广州地区甲型H1N1流感病毒的耐药情况和变异规律。结果192株中1株神经氨酸酶基因发生$247N神经氨酸酶耐药突变，对奥司他韦的敏感活性（IC）50为0。45nmol／L，比野生株下降了2．5倍。进化分析结果表明该耐药株未在广州地区流行，且与2011年初澳大利亚和新加坡流行的$247N耐药株不处于同一进化分支。结论广州地区甲型HlNl流感病毒对奥司他韦耐药的比例低，但需要加强耐药株的监测，防控来自境外输人性耐药株的传播。
Objective To study the prevalence of oseltamivir-resistance among pandemic A （HIN1） 2009 viruses isolated from patients in Guangzhou between 2009 and 2011, and to provide more information for clinical usage of oseltamivir. Methods Totally 192 pandemic A （ H1N1 ） 2009 viruses isolated from patients in Guangzhou between July 2009 and April 2011 were studies. The HA and NA genes of all strains were sequenced to reveal the evolution of viruses, and the susceptibility of viruses to oseltamivir was tested in vitro. Results One strain with a $247N mutation of the NA gene, which would make the virus resistant to osehamivir, was found. The susceptibility （IC）50 of this viral strain to oseltamivir was 0. 45 nmol/L, 2. 5 times lower as compared to the wild-type strains. Phylogenetic analysis showed that this virus was not prevalent in Guangzhou from 2009--2011, and was not located in the same branch with the strains being epidemic in Australia and Singapore during the early seasons of 2011. Conclusion The resistance rate of pandemic A（ HI NI ）2009 viruses isolated from Guangzhou to osehamivir was low, but surveillance onresistant strains needs to be strengthened to control resistant viruses imported from abroad.
出处
《中华结核和呼吸杂志》
CAS
CSCD
北大核心
2014年第1期16-20,共5页
Chinese Journal of Tuberculosis and Respiratory Diseases
基金
国家科技支撑计划课题（2012BAl05801）
国家科技重大专项子课题（重大新药创制,2013ZX09304102）
呼吸疾病国家重点实验室开放课题（2007DA780154F0903）
广东省高校科技创新重点联合攻关项目（gxzd090）
广东省教育部科技部产学研结合项目（20108091000018）
广东省科技计划项目（20108080100027）
澳门科学技术发展基金（058/2009/A2）
广州医学院附属第一医院科研基金青年项目（201220.gyfyy）
