胃癌相关下调基因在胃癌及癌前病变中的表达及其意义

Expression and clinical significance of gastric dramatic down-related gene in gastric cancer and precancerous lesions

目的观察胃癌相关下调基因(GDDR)在胃癌及癌前病变中的表达变化,探讨GDDR在胃黏膜癌变过程中的作用和意义。方法应用免疫组化SP法测定经胃镜活检和病理确诊的40例正常胃黏膜组织,40例慢性浅表性胃炎,40例慢性萎缩性胃炎,40例胃的肠上皮化生,20例轻度异型增生,20例重度异型增生和40例胃癌中的GDDR蛋白表达情况。结果从正常胃黏膜组织、慢性浅表性胃炎、慢性萎缩性胃炎、胃肠上皮化生、轻度异型增生、重度异型增生、胃癌的发展过程中,GDDR蛋白表达逐渐下调(平均秩次分别为190.70、182.70、146.24、104.40、70.33、47.20和40.20),差异有统计学意义(P<0.05)。结论 GDDR表达下调发生在胃癌形成早期,提示GDDR在胃癌的早期诊断和治疗中起重要作用,有望成为胃癌早诊的分子标记物和分子治疗的新靶点。

Objective To investigate the expression and clinical significance of gastric dramatic down-related gene （GDDR） in gastdc cancer and precancerous lesion tissues. Methods Immunohistochemical staining was performed to detect the expression of GDDR in 240 cases of gastric tissues, including 40 cases of normal gastdc mucosa （NGM）, 40 cases of chronic superficial gastritis （CSG）, 40 cases of chronic atrophic gastritis （CAG）, 40 cases of intestinal metaplasia （IM）, 20 cases of mild dysplasia （MD）, 20 cases of severe dysplasia （SD） and 40 cases of gastric cancer （GC）. Results The mean rank order of GDDR were 190. ？0, 182. ？0, 146.24, 104.40, 70.33, 47.20 and 40.20 in NGM, CSG, CAG, IM, MD, SD and GC, respectively. The expression of GDDR significantly decreased in CAG, IM, MD, SD and GC as compared with normal controls and CSG （ P 〈0.01 ）. Conclusion The gradually down-regulated expression of GDDR is an eady molecular event in the development of gastric cancer. GDDR may play an important role in the early diagnosis of gastric cancer and may be utilized as a molecular diagnostic marker and a new target of anti-tumor molecular therapy in early stage of gastric cancer.