纤维蛋白原紧密连锁不平衡基因位点BβG/A^(-455)、C/T^(-148)、G/A^(+448)与缺血性脑血管病相关性分析

Correlation of fibrinogen genetic loci BβG/A^(-455),C/T^(-148) and G/A^(+448) with close linkage disequilibrium in ischemic cerebral vascular disease

目的分析纤维蛋白原(FIB)3个紧密连锁不平衡基因位点BβG/A-455、C/T-148、G/A+448基因多态性及血浆FIB水平与缺血性脑血管病的相关性。方法以我院2010年2月至2012年2月收治的缺血性脑血管病患者89例为病例组,以同期在我院健康体检的50例为对照组,检测两组血浆FIB水平,使用多聚酶链式反应-限制性片段长度多态性(PCR-RFLP)技术测定BβG/A-455、C/T-148、G/A+448基因片段。结果缺血性脑血管病患者中,FIB基因型分布、BβA-455、T-148、A+448等位基因频率与对照组比较差异有统计学意义(P<0.05)。两组血浆FIB水平比较差异有统计学意义(P<0.01),病例组高于对照组。A、T等位基因携带者与非携带者血浆FIB水平比较差异有统计学意义(缺血性脑血管病患者组,P<0.01;对照组,P<0.05),携带者高于非携带者。结论 FIB 3个紧密连锁不平衡基因位点BβG/A-455、C/T-148、G/A+448基因多态性及血浆FIB水平与缺血性脑血管病呈明显的正相关,BβG/A-455、C/T-148、G/A+448基因多态性可能是缺血性脑血管病的遗传易感因素。

Objective To analyze the correlation between the fibrinogen gene polymorphism of three genetic loci BβG/A-455,C/T-148 and G/A＋448 with close linkage disequilibrium and the plasma fibrinogen level with the ischemic cerebral vascular disease. Methods Polymerase chain reaction restriction fragment length polymorphism（PCR-RFLP） was used to detect the gene fragments BβG/A^- 455 ,C/T ^-148 and G/A^＋448 in 89 patients with ischemic cerebral vascular disease,and 50 healthy individuals. In the meantime,plasma fibrinogen level was detected in the two groups. Results There were significant differences of genotype distributions and allele frequency BβG/A^-455, C/T^-148 and G/A^＋448 between the patients with ischemic cerebral vascular disease and the control group （ P 〈0.05）;The fibrinogen level of disease group was higher than that in the control group. Plasma fibrinogen level of allele B βA^-455 , T^-148 and A^＋448 carriers were higher than those of non carriers. The fibrinogen level of allele carriers were higher than those of the non- carriers. Conclusion An obvious positive correlation is shown between the fibrinogen genepolymorphism of three genetic loci BβG/A-455,C/T-148 and G/A＋448 with close linkage disequilibrium and the plasma fibrinogen level with the isehemic cerebral vascular disease. Gene polymorphism might be genetic susceptibility factors of ischemic cerebral vascular disease.