脐血与健康供者骨髓中血管内皮生长因子和转化生长因子β_1水平的比较

Changes of vascular endothelial growth factor and transforming growth factor-β1 in umbilical cord blood and bone marrow

目的 探讨脐血与健康供者稳态骨髓中血管内皮生长因子（VEGF）、转化生长因子β1（TGF-β1）、Tie-2、血管生成素（Ang-1、Ang-2）的血浆浓度变化.方法 采用酶联免疫吸附测定（ELISA）方法对32例脐血和健康供者稳态骨髓的VEGF、TGF-β1、Tie-2、Ang-1及Ang-2的血浆浓度进行检测.结果 脐血中VEGF的水平与稳态骨髓比较显著升高446.62（44.31-1 430.46） ng/L vs 131.62（16.62-1 201.92） ng/L（P〈0.01）.脐血中TGF-β1的水平与稳态骨髓比较却显著减低276.36（80.91-880.91） ng/L vs 476.36（235.45-1 390.00） ng/L（P＜0.01）.稳态骨髓中Tie-2的血浆浓度与脐血比较差异无统计学意义（P＞0.05）.尽管骨髓中Ang-1、Ang-2的浓度与脐血相比,有增加的趋势,但是差异均无统计学意义（P＞0.05）.结论 脐血和稳态骨髓中VEGF和TGF-β1水平的差异提示脐血和骨髓造血干细胞所处的微环境可能不同.

Objective To explore the alteration of vascular endothelial growth factor （VEGF）, transforming growth factor-β1 （TGF-β1）,Tie-2 ,angiopoietins（Ang-1 ,Ang-2） in umbilical cord blood（UCB） and bone marrow（BM）. Methods Using ELISA method,the plasma levels of TGF-β1, VEGF,Tie-2, Ang-1 and Ang-2 were detected in 32 UCB and BM donors. Results The plasma level of VEGF in UCB was significantly higher than in BM, 446.62 （44.31- 1 430.46） ng/L vs 131.62（16.62-1 201.92） ng/L（ P〈0.01）. The plasma level of TGF-131 in UCB was significantly lower than in BM,276.36（80.91-880.91）ng/L vs 476.36（235.45-1 390.00）ng/L（ P 〈0.01）. The median Tie-2,Ang- 1 and Ang-2 levels did not differ between UCB and BM samples （ P 〉0.05）. Conclusion The differences in VEGF and TGF-β1 suggest deviations in placenta and BM microenvironment.