Ki-67和pAKT在结肠癌组织中的表达

Ki-67and pAKT expression in colon carcinoma tissue

目的探讨Ki-67、磷酸化丝氨酸/苏氨酸蛋白激酶(pAKT)在结肠癌组织中的表达及与侵袭转移的关系。方法结肠癌患者72例,癌旁异型增生组织(n=72)及癌旁5cm正常黏膜组织(n=72),应用免疫组织化学链霉菌抗生物素蛋白-过氧化物酶连结(SP)法检测Ki-67、pAKT的表达。结果 Ki-67、pAKT在正常结肠黏膜组织(9.7%、9.7%)、癌旁组织(34.7%、25.0%)和结肠癌(73.6%、77.8%)组织中的阳性表达率逐渐上升(P<0.05)。晚期结肠癌组织中Ki-67、pAKT的表达明显高于早期结肠癌组织(80.3%vs 45.5%、83.6%vs 54.5%)(P<0.05),Ki-67、pAKT的表达与结肠癌在低分化组织中表达水平最高(88.9%、88.9%),高分化组织中最低(36.4%、45.5%)(P<0.05)。Ki-67、pAKT在淋巴结转移组中的表达明显高于无转移组(80.0%vs 18.5%、86.7%vs 33.3%)(P<0.05)。结论 Ki-67、pAKT异常表达与结肠癌的发生、发展密切相关,可作为临床参考指标。

Objective To investigate the expression of nuclear proliferation index（Ki-67） and serine/threonine protein kinase（pAKT） in colon cancer metastasis and their relations between invasion and biological behavior. Methods Seventy-two patients with colon cancer served as observation group while the adjacent dysplasia tissues（ n = 72） and normal mucosa 5 cm adjacent the cancer（ n =72） in the 72 cases were put as control group. Immunohistochemical SP method was used to detect the expression of Ki-67 and pAKT. Results The positive expression rate of Ki-67 ,pAKT in normal colon mucosa （9.7%,9.7%）, tissue adjacent to the cancer （34.7%, 25.0%） and colon cancer （73. 6%, 77.8%） gradually increased （P 〈 0.05）. The expressions of Ki-67 and pAKT in advanced colon cancer were significantly higher than those of early-stage colon cancer （80.3~ vs 45.5%, 83.6% vs 54.5%） （ P 〈0.05）. The expressions of Ki-67 and pAKT were the highest in poorly differentiated colon cancer tissues （88.9 %,88.9 % ）, while the expression levels were the lowest in well differentiated tissue（36.4% ,45.5%）（ P〈0.05）. The expressions of Ki 67 and pAKT in lymph node metastasis group were significantly higher than those without metastasis （80.0% vs 18.5% ,86.7% vs 33.3%）（ P 〈0.05）. Conclusion Ki-67 and pAKT abnormal expression were closely related to the development of colon cancer and they could be used as clinical indexes.