摘要
目的观察鼠脑胶质瘤组织中调节性T细胞(Treg)的表达变化,并探讨其对脑胶质瘤的作用。方法①建立9L/Fisher344大鼠脑胶质瘤模型,分别于造模后7、14、21 d和临终状态时处死大鼠,获取胶质瘤组织及对侧正常脑组织标本,行流式细胞学检测,观察Treg细胞比例变化。②将Fisher344大鼠随机分为实验组和对照组各15只,实验组在造模前3 d腹腔内注射抗CD25单克隆抗体(PC61),对照组注射相同剂量的IgG-1。两组在造模后14 d分别处死5只大鼠,比较两组胶质瘤中Treg细胞的浸润情况,剩余大鼠观察生存时间。结果鼠脑胶质瘤组织中浸润的Treg细胞比例明显高于正常脑组织(P<0.01),并随时间延长而增高,到临终时达到高峰。应用PC61后,实验组脑胶质瘤中Treg细胞比例明显低于对照组(P<0.05),且大鼠的生存时间明显长于对照组(P<0.05)。结论Treg细胞在鼠脑胶质瘤组织中的表达明显升高,清除Treg细胞能明显抑制鼠脑胶质瘤的生长;Treg细胞在胶质瘤生长过程中发挥重要作用。
Objective To observe the expression changes of regulatory T （Treg） cells in the rat glioma tissues and to explore its role in glioma. Methods ① After the establishment of 9L/Fisher344 rat brain glioma models, rats were killed at day 7, 14, 21 and at the final stage. Subsequently, the tissues of glioma and normal brain were isolated and analyzed for the presence of Treg cells by flow cytometry. ② The Fisher344 rats were randomly divided into the experimental group and the control group with 15 rats in each. Rats in the experimental group were intraperitoneally injected with anti-CD25 mAbs （ PC61 ） 3 d before tumor inoculation, while rats in the control group were injected with IgG-1. Five rats from each group were killed at day 14 after inoculation, and the infiltration of Treg cells was analyzed by flow cytometry. The survival time was observed for the remaining rats. Results The infiltration proportion of Treg cells in the rat glioma tissues was signifi- cantly higher than that in the normal brain tissues （P 〈 0.01 ）, increased with the time and reached a peak at the death- bed. After application of PC61, the Treg cells in the glioma of experimental group were significantly lower than that of the control group （P 〈 0.05）, and survival time was significantly longer than that of the control group （P 〈 0.05 ）. Conclu- sion The expression of Treg cells in the rat glioma tissues is significantly elevated, clearing Treg cells can inhibit the growth of rat glioma and Treg cells play an important role in the process of glioma growth.
出处
《山东医药》
CAS
2014年第3期17-19,共3页
Shandong Medical Journal
基金
河北省医学科学研究重点课题计划(20110470)
