1株拮抗水稻纹枯病菌鼠乳杆菌的筛选与鉴定

Screening and Identification of a Lactobacillus murinus Strain to Antagonize Paddy Pattern Blight( Rhizoctonia solani)

采用平板对峙法从浙江省金华市水稻田土壤中筛选获得1株拮抗水稻纹枯病菌的细菌QT-0304菌株,最大拮抗带宽达到20 mm。根据形态特征、生理生化特征、16S rRNA基因比对及进化树构树分析,鉴定QT-0304菌株为鼠乳杆菌(Lactobacillus murinus)。抗菌谱实验结果表明:QT-0304菌株对苹果腐烂病菌(Valsa mali)和杨树溃疡病病菌(Botryosphaeria dothidea)均有较好的抑制作用,拮抗带宽分别达到34.5 mm和23.0 mm。

A bacterial strain QT-0304 antagonizing paddy pattern blight （ Rhizoctonia solani） was obtained from paddy field soil in Jinhua, Zhejiang Province, adopting flat plate confrontation method, its maximum antagonistic band width was up to 20 mm. According to its morphological features, physiological and biochemical characteristics, 16S rRNA gene sequence comparison as well as genealogical tree construction analysis, the strain QT-0304 was identified as Lac-tobacillus murinus. The antibacterial spectrum experimental results showed that strain QT-0304 has fairly good inhibi-tion against apple decaying disease （Valsa mali） and poplar ulcer （Botryosphaeria dothidea）, and the antagonistic band width could reach up to 34. 5 mm and 23. 0 mm, respectively.