梓醇对永久性脑缺血大鼠恢复早期实时步态的影响

Influence of Catalpol on Real-Time Gait Analysis of Cerebral Ischemia in Rats

目的：观察梓醇对pMCAO大鼠恢复期早期实时步态的影响，评价其对缺血性卒中的改善作用。方法：将大鼠随机分为6组，即正常对照组，模型组，阳性药天保宁组，梓醇15、30、60 mg＆#183;kg-1组。于术前进行为期7天的步态仪行走训练，每3次。训练完成后，采用线栓法建立永久性大脑中动脉栓塞（pM原CAO）大鼠模型，并于术后3-14天连续给药，术后第14天进行实时步态行为检测。结果：术后14天，与正常组比较，pMCAO模型大鼠平均步行周期显著延长（P〈0.01），绝对值平均体转角明显升高（P〈0.05），双足支撑时长缩短（P〈0.05），三足支撑时长延长（P〈0.01），协调性指标显著增加（P〈0.01）。与模型组比较，梓醇30 mg＆#183;kg-1及60 mg＆#183;kg-1组模型动物步行周期、降低绝对值平均体转角显著缩短（P〈0.05或P〈0.01）；梓醇30 mg＆#183;kg-1组右前相对于其他三足的协调性指数明显降低（P〈0.05），脑缺血动物协调性提高。结论：梓醇可部分改善脑缺血模型大鼠的实时步态行为变化，对缺血性卒中的神经功能有一定保护作用。

This study was aimed to observe the influence of Catalpol on real-time gait analysis of early recovery after permanent middle cerebral artery occlusion （pMCAO） in rats to evaluate its effect on the improvement after cerebral ischemia. Rats were randomly divided into 6 groups, which were the normal control group, model group, Tianbaoning group, Catalpol 15, 30, 60 mg＆#183;kg-1 group. Rats were trained on the gait instrument for 7 days before pMCAO, 3 times/day. After the training, pMCAO model was made. And continuous infusion was performed from the 3rd to the 14th day after the operation. Then, the real-time gait behavior was detected on the 14th day. The re-sults showed that 14 days after the surgery, compared with the normal control group, the models had a significant extending in the duty cycle （P 〈 0.01）, and obvious increasing of the average body angle of absolute value （P 〈0.05）, the shortening of the two feet supporting time （P 〈 0.05）, and extending of three feet supporting time （P 〈0.01）, and increasing of the coordination index （P 〈 0.01）. Compared with the model group, the Catalpol of 30 mg＆#183;kg-1 and 60 mg＆#183;kg-1 group has obviously decreased duty cycle and average body angle of absolute value （P 〈0.05, or P 〈 0.01）. The Catalpol of 30 mg＆#183;kg-1 can obviously reduce the coordination index of the right front foot relative to the other three feet （P 〈 0.05）, which improved the coordination of cerebral ischemia animals. It was concluded that Catalpol can improve the real-time gait behavior changes of cerebral ischemia model rats. There-fore, Catalpol have a neural protective effect on cerebral ischemia.