益气消法对雌激素诱导子宫肌瘤模型豚鼠血管内皮生长因子及其受体蛋白表达的影响

Influence on Angiogenesis Factor and Expression of Its Receptor Protein of Estrogen-induced Uterine Fibroids Rat Model with Yi-Qi Xiao-Zheng Method

目的:观察益气消癥法对雌激素诱导子宫肌瘤模型豚鼠血管内皮生长因子及其受体蛋白表达的影响,探讨该法方药抑制血管生成治疗子宫肌瘤的作用机制。方法:通过去势+皮下注射苯甲酸雌二醇建立子宫肌瘤模型,并给予不同剂量益气消癥法中药进行治疗。采用免疫组化法检测子宫组织微血管密度(MVD)、血管内皮生长因子(VEGF)蛋白表达水平;采用RT-PCR检测子宫组织VEGFR1 mRNA、VEGFR2mRNA表达。并对以上指标进行统计学分析。结果:与正常对照组比较,模型组豚鼠子宫肌组织MVD、VEGF、VEGFR表达显著升高(P<0.01);与模型组比较,中药各剂量组均有不同程度的降低,其中以高剂量组最为明显,说明益气消癥法高剂量中药组可以显著抑制MVD、VEGF及其受体的蛋白表达。结论:益气消癥法通过抑制血管生成进而抗肌瘤细胞增殖,这可能是该法治疗子宫肌瘤的作用机制之一。

This study was aimed to observe the influence on angiogenesis factor and expression of its receptor protein on estrogen-induced uterine fibroids rat models with Yi-Qi Xiao-Zheng （YQXZ） method, in order to explore the mechanism of this method in the inhibition of angiogenesis in the treatment of uterine fibroids. Castration and estro- gen-induced method were used in the establishment of guinea pig uterine fibroids rat model. And then, different doses of Chinese medicine of YQXZ method were given. The immunohistochemical method was used in the detection of microvessel density （MVD） and vascular endothelial growth factor （VEGF） protein expression of the uterine tissues. The RT-PCR method was used to detect the expression of VEGFR1 mRNA and VEGFR2 mRNA. Then, statistical analysis was given on indexes mentioned above. The results showed that compared with the normal control group, ex- pression of MVD, VEGF and VEGFR of uterine muscle tissues in the model group was significantly higher （P 〈 0.01）. Compared with the model group, Chinese medicine group of different doses showed different reducing. Among them, the high-dose group was the most obvious one. It showed that the high-dose Chinese medicine of YQXZ group was able to significantly inhibit the expression of MVD, VEGF and theirs receptors. It was concluded that YQXZ method can antagonize proliferation of the leiomyoma cell by inhibiting angiogenesis. Therefore, it may be one of the mechanisms of this method in the treatment of uterine fibroids.