系统性红斑狼疮患者CD4^+CD25^+Treg细胞中Ets-1的表达

Expression of Ets-1 mRNA in CD4^+CD25^+T regulatory cells from patients with systemic lupus erythematosus

目的研究系统性红斑狼疮(SLE)患者CD4+CD25+Treg细胞中E26转录因子-1(Ets-1)mRNA的表达水平,探讨其表达变化与SLE发病的相关性。方法收集30例SLE患者及健康者的抗凝静脉血20 ml,采用磁珠分选试剂盒分选出CD4+CD25+Treg细胞,实时荧光定量PCR技术检测Treg细胞中Ets-1 mRNA的表达水平,分析其与SLE疾病活动指数(SLEDAI),以及主要临床表现和实验室指标之间的关联。结果 SLE组CD4+CD25+Treg细胞中Ets-1 mRNA表达水平[0.225(0.111,0.416)]低于健康对照组[0.503(0.248,0.785)](P=0.011);根据SLEDAI评分将SLE患者分为疾病活动组和稳定组,疾病活动组Ets-1 mRNA表达水平[0.190(0.121,0.617)]低于稳定组[0.243(0.094,0.304)],但差异无统计学意义(P=0.895);与健康对照组相比,疾病活动组和疾病稳定组Ets-1 mRNA表达水平均降低(P<0.05)。SLE患者Ets-1 mRNA的表达水平与SLEDAI、主要临床表现和实验室指标之间均无相关性。结论SLE患者CD4+CD25+Treg中Ets-1 mRNA表达水平显著降低,提示CD4+CD25+Treg中Ets-1的表达异常可能与SLE的发病有关。

Objective To detect the mRNA expression of Ets-1 mRNA in CD4^ ＋CD25^ ＋ Treg cells from patients with systemic lupus erythematosus （SLE）, in order to investigate its potential role in the pathogenesis of SLE. Methods Peripheral blood from SLE patients and healthy controls was collected, respectively. PBMCs were fur-ther purified using CD4^ ＋CD25^ ＋ regulatory T Cell Isolation Kit. Real-time transcription-polymerase chain reaction analysis （ RT-PCR） was performed to determine the expression of Ets-1 mRNA in CD4^ ＋CD25 ^＋ Treg cells. The correlations between Ets-1 mRNA and systemic lupus erythematosus disease activity index （ SLEDAI ） , laboratory measurements, clinical features were also analyzed. Results Compared with the healthy controls [0.503（0.248, 0.785 ） ] , the Ets-1 mRNA expression level was decreased in patients with SLE [ 0.225 （ 0.111 , 0.416 ） ] （ P=0.011 ） . SLE patients were separated into active SLE groups and inactive SLE groups according to the SLEDAI. No significant difference was found between active SLE groups [ 0.190 （ 0.121 , 0.617 ） ] and inactive SLE groups [0.243（0.094, 0.304）] （P =0.895）. Compared to healthy controls, Ets-1 mRNA expression in active SLE groups and inactive SLE groups were decreased （ P＆lt;0.05 ） . No correlations were found between Ets-1 mRNA and SLEDAI, laboratory measurements, clinical features. Conclusion The expression levels of Ets-1 mRNA in CD4 ＋CD25 ＋ Treg cells from patients with SLE are significantly decreased. The results indicate that the abnormal expres-sion of Ets-1 may contribute to the pathogenesis of SLE.