斑鳢、乌鳢及其杂种细胞核DNA流式含量分析

Cellular DNA Contents of Channa maculata,C. argus and Their Reciprocal Hybrids Determined by Flow Cytometry

以斑鳢(Channa maculata)、乌鳢(C.argus)及其正交杂种斑乌鳢(斑鳢♀×乌鳢♂)和反交杂种乌斑鳢(乌鳢♀×斑鳢♂)的红细胞为材料,以鸡(Gallus gallus)血细胞为DNA标准(2.5 pg/2c,2c指2倍体),采用流式细胞仪测定了这4种鱼的细胞核DNA含量。斑鳢、乌鳢、斑乌鳢及乌斑鳢这4种鱼血细胞DNA的绝对含量分别为(1.488±0.035)pg/2c、(1.489±0.034)pg/2c、(1.522±0.077)pg/2c和(1.520±0.033)pg/2c。斑鳢和乌鳢的细胞核DNA含量差异不显著(P>0.05),斑鳢和乌鳢与两种杂交鳢的DNA含量差异显著(P<0.05),两种杂交鳢之间的细胞核DNA含量差异不显著(P>0.05)。杂交鳢细胞核DNA含量显著高于亲本,可以作为杂种鉴定的依据。

The cellular DNA contents of Channa maculata, C.argus and their reciprocal hybrids （C.maculata ♀×C.argus ♂, C.argus ♀×C.maculata ♂） were assayed by using a flow cytometer with the DNA of chicken red blood cells （2.5 pg/2c, 2c：diploid） as a standard. The DNA contents of the 4 species of fish are 1.488±0.035 pg/2c （C.maculata ♀）, 1.489±0.034 pg/2c （C.argus ♂）, 1.522±0.077 pg/2c （C.maculata ♀×C.argus ♂）, and 1.520±0.033 pg/2c （C.argus ♀×C.maculata ♂） respectively. Comparing the DNA contents of the 4 species of fish, it was showed that there was no significant difference between C.maculata and C.argus （P〉0.05）, and the similar result was obtained between their reciprocal hybrids （P〉0.05）, but there was significant difference between reciprocal hybrids and their parents （P〈0.05）. The DNA contents of the reciprocal hybrids are more than those of their parents, which may be used for hybrid identification.