PIWIL1在肝细胞癌中的表达及临床意义

Clinical significance of expression of PIWIL1 protein in hepatocellular carcinoma

目的:探讨干细胞相关蛋白PIWI样蛋白1(PIWI-like protein-1,PIWIL1)在肝细胞癌组织中的表达及临床意义,并探索其表达与患者预后的关系.方法:应用免疫组织化学检测47例肝细胞癌标本中PIWIL1的表达,免疫印迹(Western blot)法检测31例肝细胞癌组织中PIWIL1的表达,分析PIWIL1表达与临床特征及预后的关系.结果:PIWIL1在肝癌组织中高表达,在正常肝组织中低表达,PIWIL1同肿瘤分化程度、有无临近组织侵犯有关,与肿瘤大小及甲胎蛋白(α-fetoprotein,AFP)水平无关;其阳性表达预示患者预后较差.结论:PIWIL1的高表达同病理分化程度、临近组织侵犯相关及患者预后相关.

AIM： To discuss the clinical significance of positive expression of PIWI-like protein-1 （PIWIL1） in hepatocellular carcinoma （HCC）. METHODS： Immunohistochemistry was used to detect the expression of PIWIL1 in 47 cases of HCC and matched tumor-adjacent hepatic tissues. Western blot was used to detect the expression of PIWIL1 in 31 fresh HCC tissues and matched tumor-adjacent hepatic tissues. The relationship between PIWIL1 positive expression and clinical features or prognosis of HCC patients was analyzed. RESULTS： The positive rate of PIWIL1 expression was significantly higher in HCC than in normal liver tissue （P 〈 0.05）. PIWIL1 expression was positively correlated with the invasion to the adjacent tissue and the histological differentiation （both P 〈 0.05）. PIWIL1 positive cases had a poor prognosis. CONCLUSION： The high expression of PIWIL1 is related to pathological differentiation and the invasion to the adjacent tissue, as well as to the prognosis of HCC patients.