胸腹水细胞块制作技术及应用

Pleural Celiac Fluid Cell Blocks Making and Application

目的探讨用琼脂作为支架制作胸腹水细胞块的技术、细胞块及免疫细胞化学染色技术在细胞病理学诊断中的应用价值。方法临床送检的胸腹水离心,取沉淀物做成细胞块包埋制片,同时进行免疫细胞化学染色。结果细胞块组的阳性率(35/75,46.7%)明显高于常规涂片组(25/75,33.3%),同时利用细胞块进行细胞免疫组化实验,帮助鉴别诊断,大大提高了阳性检出率(49/75,65.3%)。结论细胞块制作简单、方便,大大提高了诊断的准确性,对胸腹水的诊断具有较高的的实用价值。

Objective To evaluate the value of making pleural and celiac fluid into cell blocks with agar as the bracket, cell blocks and irrtmunohistochernistry in the application of cytopatbology diagnosis. Methods Clinical cases ofpleural and celiac fluid were made into cell blocks by centrifugal separation and taking precipitate, irnmunocytochemical was carried out as well. Results The positive rate of cell blocks group （35/75, 46.7%） was obviously higher than that of conventional smear group （25/75, 33.3%） and the immunocytochernistry experiments with cell blocks assisted antidiastole greatly improving the positive rate （49/75, 65.3%）. Conclusions As simplicity and convenience, as well as great improvement in the accuracy of diagnosis, cell blocks are of great value to diagnosis ofpleural and celiac fluid.