DART-Orbitrap质谱法快速筛查饲料中磺胺类药物

Rapid Screening of Sulfonamides Drugs in Animal Feed Using DART-Orbitrap High Resolution Mass Spectrometry

利用新型离子化技术——实时直接分析（DART）离子源与高分辨质谱Orhitrap联用，建立饲料中非法添加磺胺类药物的快速筛查方法。通过对DART离子化温度、样品检测模式等参数进行探索优化，最终确定饲料经过乙腈一水溶液简单提取，液体筛网模块进样，450℃下离子化，Orhitrap高分辨质谱在全扫捕模式下定性检测，并进行相关的方法学验证，方法检出限为1．O～4．0μg／g。该方法前处理简单、检测速度快、定性准确、样品通量大、环保无污染，能满足饲料中非法添加磺胺类药物快速筛查的要求。

By applying an amb tandem Orbitrap MS, a rapi confirmation of sulfonamides lent d sc lOnlzatl reenmg on-direct analysis in real time （DART） ion source method was developed for the determination and drugs in animal feed. Through optimization of ionization tern perature and sample injection modu were extracted using 10 mL mixtur mission module, ionized under 450 scan mode. The results show that Orbitrap MS method can be used fo added in animal feed. [es e of ℃ the the screening method was established. water and acetonitrile（3： 7,V/V） inje and then analyzed using DART-Orbitr r rap imit of dete d and direct ction is 1.0-4. 0 μg/g. analysis of sulfonamides Feed samples cted by ap MS trans- in full This DART- drugs illegally