“温阳补气”针法对实验性自身免疫性重症肌无力大鼠血清AchR-Ab和IFN-γ表达水平的影响及其作用机制

Influence of “Warming yang and Nourishing qi”acupuncture treatment in serum AchR-Ab and IFN-γexpression levels in rats with experimental autoimmune myasthenia gravis and its mechanism

目的:观察"温阳补气"针法对实验性自身免疫性重症肌无力(EAMG)大鼠血清中乙酰胆碱受体抗体(AchR-Ab)和干扰素γ(IFN-γ)表达水平的影响,分析针灸治疗重症肌无力(MG)的作用机制。方法:采用乙酰胆碱受体α1(AchRα1)129-145多肽片段免疫接种雌性Lewis大鼠,建立EAMG模型。随机选取建模成功的EAMG大鼠30只,分为针灸治疗组、药物对照组及模型对照组,每组10只,并将同期购进未建模的10只大鼠设为空白对照组。针灸治疗组大鼠予以"温阳补气"针法治疗,每次30min,每天1次,7d为一疗程,疗程间休息1d,连续治疗2个疗程;药物对照组大鼠予以溴吡斯的明灌胃治疗,18.5mg·kg-1·d-1,连续治疗15d;其余2组不予任何特殊处置,仅作对照观察。治疗前后24h,抽取大鼠尾根静脉血,采用ELISA法测定大鼠血清中AchR-Ab和IFN-γ的表达水平。结果:与模型对照组及治疗前比较,治疗后针灸治疗组和药物对照组EAMG大鼠血清的AchR-Ab和IFN-γ表达水平均明显降低(P<0.05或P<0.01);与空白对照组比较,模型对照组大鼠血清中AchR-Ab和IFN-γ表达水平明显升高(P<0.01)。但治疗后针灸治疗组与药物对照组比较无明显变化(P>0.05)。结论:"温阳补气"针法可以降低EAMG大鼠血清中AchR-Ab和IFN-γ的表达水平,从而达到治疗MG的作用。

Objective To observe the influence of the ＂Warming yang and Nourishing qi＂ acupuncture treatment in the serum acetylcholine receptor antibody （AchR-Ab） and interferon-gamma （IFN-γ） expression levels in the rats with experimental autoimmune myasthenis gravis （EAMG） and to analyze the mechanism of acupuncture treatment to myasthenis gravis （MG）. Methods AchRal 129-145 peptide fragments were used to immune the female Lewis rats to establish EAMG models. 30 successful EAMG model rats were randomly selected and divided into acupuncture treatment group, drug control group and model control group （n = 10）; the unmodelled 10 ratspurchased at the same period were used as blank control group. The rats in acupuncture treatment group were treated with ＂Warming yang and Nourishing qi＂ acupuncture treatment, 30 min per time, once a day, 7 d for a period, 1 d rest between two periods, two periods of continuous treatment. The rats in drug control group were treated with pyridostigmine lavaged treatment, 18.5 mg·kg-1·d-1, continuous treatment of 15 d. The rats in the other two groups had no any special treatment. 24 h before and after treatment, the blood samples from the vein of tail root of the rats in 4 groups were collected, and the serum expression levels of AchR-Ab and IFN-γ were detected with ELISA method. Results Compared with model control group and before treatment, the serum AchR- Ab and IFN-γ expression levels of the EAMG rats in acupuncture treatment and drug control group after treatment were significantly decreased （P〈0.05 or P〈0.01） ; compared with blank control group, the serum AchR-Ab and IFN-γ expression levels of the rats in model control group were significantly increased （P〈0.01）; but there was no significant difference between acupuncture treatment group and control group after treatment （P 〉0.05）. Conclusion ＂Warming yang and Nourishing qi＂ acupuncture treatment can reduce the serum AchR-Ab and IFN-γ expression levels of the EAMG rats to treat MG.