藏药甘肃蚤缀脂溶性成分的GC-MS分析

GC-MS Analysis of the liposoluble constituents from Arenaria kansuensis Maxim.

研究藏药甘肃蚤缀的脂溶性成分,进一步揭示该药材的化学成分,为这一传统藏药资源的开发利用提供实验依据。采用95%乙醇回流提取甘肃蚤缀全草,滤液浓缩后用石油醚萃取,得到脂溶性部位,通过气相色谱-质谱联用技术分析其化学成分,结合计算机检索技术及美国国家标准技术研究所(NIST)化合物谱库检索进行鉴定,采用面积归一化法确定其相对含量。从甘肃蚤缀石油醚部位共分离鉴定出25个化合物,相对含量占总含量的91.31%,包括脂肪类、甾体、三萜及芳香化合物等。

The first study was to explore the chemical compounds of the liposoluble parts from Arenaria kansuensis Maxim,and pro-vide the test basis for further rational expoitation and utilization of its medicinal resources. The air-dried whole plants of Arenaria kansuensis Maxim. were extracted with 95% ethano1. The extract was concentrated and extracted with petroleum ether to get the li-posoluble parts,and the chemical composition was identified and analyzed by GC-MS method. Combining with computer retrieval technology and search of national institute of standards and technology（ NIST） compound spectral-library for identification,their rela-tive content was determined by the area normalization method. Totally 25 compounds were identified and their corresponding relative contents were evaluated by area normalization method. The content of all identified compounds is 91. 31%,of them,most are ali-phatic derivatives,steroids,triterpene and aromatic compounds.