肝癌患者血清microRNA-21水平变化

Serum level of microRNA-21 in patients with hepatocellular carcinoma

目的分析不同肝病患者血清microRNA-21(miR-21)水平,以探讨miR-21对肝细胞癌(HCC)的诊断价值。方法以实时定量逆转录PCR法检测正常人、慢性乙型肝炎(CHB)、肝硬化和HCC患者(各组均为25例)血清miR-21水平,分析miR-21水平与HCC临床病理学特征的关系。结果正常人、CHB和肝硬化患者血清miR-21相对水平分别为(1.1±1.7)、(2.3±2.6)和(2.8±2.5),而HCC患者为(22.6±4.4),显著高于前三组(P<0.001);HCC患者术后1w和1m血清miR-21相对水平分别为(18.4±3.5)和(3.1±2.7),均较术前显著降低(P<0.001);HCC患者血清miR-21水平与肿瘤大小、癌栓以及HBV感染相关,与肿瘤分化程度、数目和血清AFP水平无相关性。结论 miR-21在HCC患者血清中显著升高,可能作为HCC早期诊断的潜在标志。

Objective To evaluate serum level of microRNA-21 （miR-21） in normal persons and patients with different liver diseases,and to investigate its effect in the diagnosis of hepatocellular carcinoma（HCC）. Methods The serum level of miR-21 in healthy controls （n=25）,patients with chronic hepatitis B（CHB,n=25）, cirrhosis （n=25）,and HCC （n=25） were measured by real-time quantitative RT-PCR. The relationships between serum miR-21 levels and clinical pathological features of HCC were also investigated. Results The relative serum levels of miR-21 in normal controls,CHB and cirrhotic patients were （1.1±1.7）,（2.3±2.6） and （2.8±2.5）,respec-tively,all of which were significantly lower than that in HCC patients（22.6±4.4）,P〈0.001;The relative serum levels of miR-21 in HCC patients one week and one month after surgery were（18.4±3.5） and （3.1±2.7）,respectively,sig-nificantly lower than that before operation （22.6±4.4）,P〈0.001;Serum levels of miR-21 were correlated with tumor size,tumor thrombosis and HBV infection,but not with tumor numbers,tumor differentiation and serum AFP levels. Conclusions Serum levels of miR-21 is elevated in HCC patients and might be used as a potential biomarker for the early diagnosis of HCC.