疏风解毒胶囊体内抗病毒作用的实验研究

In-vivo Experimental Research of Antiviral Action of Shufeng Jiedu Capsule

目的观察疏风解毒胶囊体内抗呼吸道合胞病毒(RSV)、单纯疱疹病毒I型(HSV-I)和柯萨奇病毒B3型(CVB3)的作用。方法分别用RSV、HSV-I、CVB3感染小鼠建立模型,实验分疏风解毒胶囊高、中、低剂量组,利巴韦林组,清开灵组,病毒对照组及正常对照组,灌胃给药,记录各组小鼠死亡数及存活时间。以肺指数、动物死亡保护率及生命延长率等指标观察疏风解毒胶囊体内抗病毒的作用。结果与病毒对照组比较,疏风解毒胶囊能明显降低RSV感染小鼠肺指数,并存在剂量依赖关系,其肺指数抑制率略低于利巴韦林组,而明显高于清开灵组。同时疏风解毒胶囊对3种病毒所致的动物死亡保护率、生命延长率等指标均明显增加,与上述对肺指数抑制率的结果吻合。结论疏风解毒胶囊可有效地改善RSV感染所引起的小鼠肺部肿胀,对RSV、HSV-I、CVB3病毒感染小鼠均有明显保护作用。

objective To observe the anti-viral effects of Shufeng Jiedu capsule on mice models infected with respiratory syncytial virus（RSV）, herpes simplex virus type I（HSV-I） and coxsackie virus B3 type（CVB3） respectively. Methods Mice models of RSV, HSV-I or CVB3 infection were established. High dose, moderate dose and low dose of Shufeng Jiedu capsule were administrated intragastrically to the model mice, and Ribavirin and Qingkailing granula were used as positive control medicine. The uninfected mice served as normal control. Mice survival time and the number of surviving mice were recorded, and the antiviral activity of Shufeng Jiedu capsule was evaluated by lung index, the rate of death protection and lifespan extension. Results In comparison with the viral infection model group, Shufeng Jiedu capsule significantly reduced the increase of lung index of mice with RSV infection in a dose-dependent manner, and its inhibitory rate for lung index was slightly lower than that of Ribavirin particles, but significantly higher than that of Qingkailing particles. The death protection rate and lifespan extension rate of three kinds of mice model were increased after treatment with Shufeng Jiedu capsule, and the results were in accordance with the inhibitory rate for lung index. Conclusion Shufeng Jiedu capsule can significantly relieve the pulmonary edema in RSV-infected mice, and has protective effect on mice infected with RSV, HSV-I, CVB3.