沉香挥发油对H_2O_2致PC12细胞氧化损伤的保护作用

Protective Effects of Lignum Aquilariae Resinatum Essential Oil on H_2O_2-induced Oxidative Damage of PC12 Cells

目的研究沉香挥发油对H2O2所致鼠肾上腺嗜铬细胞瘤单克隆细胞系(PC12细胞)氧化损伤的保护作用。方法体外培养PC12细胞,采用H2O2制备细胞损伤模型,运用乳酸脱氢酶(LDH)和四唑盐(MTT)法检测细胞活力;应用荧光分光光度法测定细胞膜电位(MMP)和细胞内活性氧含量;应用试剂盒-酶标仪法测定细胞内丙二醛(MDA)含量、超氧化物歧化酶(SOD)和谷胱甘肽还原酶(GSH-Px)的活力。结果不同浓度H2O2对PC12细胞生长均有明显的抑制作用,当浓度达到200μmol·L-1后,对细胞的抑制作用明显减缓;与正常对照组对比较,模型组的细胞存活率较低,ROS、MDA含量较高(P<0.01),但SOD和GSH-Px含量明显降低(P<0.01);与模型组比较,沉香挥发油各剂量组的ROS、MDA含量明显降低(P<0.01),而细胞存活率、SOD和GSH-Px酶活力则显著增强(P<0.01)。结论沉香挥发油在一定剂量范围内,对H2O2致PC12细胞氧化损伤具有保护作用。

Objective To study the protective effects of Lignum Aquilariae Resinatum essential oil on the H2OE- induced oxidative damage of pheochromocytoma monocloned cell line （PC12 cells）. Methods PC12 cells was cultured in vitro, and oxidative damage of PC12 cells was induced by H202. MTF method was performed for the cletection of cells viability, fluorospectrophotometry was used to determinate mitochondrial membrane potential （MMP） and the content of intracellular reactive oxygen species（ROS）, and the kit-microplate reader methods were used to determinate malondialadehyde（MDA） content and the activities of superoxide dismutase（SOD） and glutathione reductase（GSH-Px）. Results Each concentration of H202 had significant inhibition on PC12 cells, and 200 μmol. L^- 1was the maximum inhibitory concentration. The cell viability of the model group was lower, while the contents of ROS and MDA were higher than those of the blank control group（P 〈 0.01）, and the activities of SOD and GSH-Px were sharply reduced in the model group （P 〈 0.01 ）. After being treated with Lignum Aquilariae Resinatum essential oil, ROS and MDA were decreased, and the cell viability and the activities of SOD and GSH-Px were increased（P 〈 0.01 compared with the model group）. Conclusion Lignum Aquilariae Resinatum essential oil has protective effect on oxidative damage of PC12 cells in its effective dose range.