红背叶根不同提取物体外抑制HBsAg和HBeAg的实验研究

Experimental Study of Radix Alchorneae Trewioidis Extracts on Inhibiting HBsAg and HBeAg in vitro

目的观察红背叶根4种不同提取物对HepG 2.2.15细胞分泌HBsAg和HBeAg的影响,探讨红背叶根不同提取物体外抗HBV作用。方法通过四甲基偶氮唑盐(MTT)法观察红背叶根水提取物(WE)、石油醚提取取物(EE)及正丁醇提取物(BE)对HepG 2.2.15细胞的影响,采用ELISA法检测分析红背叶根4种提取物对HepG 2.2.15细胞分泌HBsAg和HBeAg的抑制作用。结果红背叶根正丁醇提取物在无毒浓度下对HepG2.2.15分泌的HBsAg、HBeAg的抑制率分别达65.2%、94.4%,治疗指数分别为>9.8和>67.1;红背叶根乙酸乙酯提取物对HBeAg抑制率达53.4%,治疗指数>2,而对HBsAg的无抑制作用;其他两种提取物对HBsAg、HBeAg则无抑制效果。结论红背叶根乙酸乙酯提取物、正丁醇提取物均有抗HBV作用,正丁醇提取物效果比乙酸乙酯效果更好,具有潜在的抗HBV开发前景。

Objective To observe the effect of four kinds of extracts from Radix Alchorneae Trewioidis on the secretion of HBsAg and HBeAg from HepG2.2.15 cells, and to investigate the effect of Radix Alehorneae Trewioidis extracts against HBV. Methods The dried Radix Alehorneae Trewioidis powder was dissolved in distilled water, and then was extracted with petroleum ether, ethyl acetate, n-butanol alcohol in order for 2 times. Water extract（WE）, petroleum ether extract （PE）, ethyl acetate extract （EE）, n-butanol extract （BE） were obtained respectively. M3T assay was used to observe the effect of different Radix Alchorneae Trewioidis extracts on HepG2.2.15 cell, and ELISA assay was used to detect the inhibition on HBsAg and HBeAg secretion from HepG2.2.15 cells. Results The inhibition rate of Radix Alchorneae Trewioidis BE on the secretion of HBsAg, HBeAg from HepG 2.2.15 reached 65.2 %, 94.4 % respectively at non-toxic concentrations, and the therapeutic index was over 9.8, 67.1 respectively. The inhibition rate of Radix Alchorneae Trewioidis EE on HBeAg was 53.4 %, the therapeutic index was over 2, while Radix Alehorneae Trewioidis EE had no inhibitory effect on HbsAg. Radix Alchorneae Trewioidis WE and PE had no effect on the inhibition of HBsAg and HBeAg. Conclusion Both BE and EE of Radix Alchorneae Trewioidis can inhibit HBV in vitro, and the effect of BE is stronger, showing potential development prospects against HBV.