毛冬青对心衰模型大鼠心功能及miR133a表达的影响

Effect of Radix Ilecis Pubescentis on Heart Function and the miR133a Expression in Chronic Heart Failure Rats

目的观察毛冬青对心衰大鼠心功能及微小RNA133a(miR133a)表达的影响。方法腹主动脉缩窄术复制大鼠心衰模型,随机分为毛冬青高、低剂量组,卡托普利组,心衰模型组和假手术组,干预2周后抽血查B型尿钠肽(BNP)和行心脏彩超检查心功能,并对大鼠心脏组织miR133a进行荧光PCR检测。结果心衰模型组的心功能指标明显低于假手术组,3个药物干预组心功能指标均有较好的提高作用,组间作用无显著性差异;与假手术组比较,心衰模型组miR133a的表达下调,各药物干预组与心衰模型组比较,miR133a的表达均显著上升,其中以毛冬青低剂量组最为显著。结论腹主动脉缩窄可成功复制大鼠慢性心力衰竭模型,心力衰竭大鼠模型较假手术组大鼠miR133a的表达下调。不同剂量的毛冬青均能改善慢性心力衰竭大鼠模型的心功能,并且能上调miR133a的表达,但上调幅度与心功能改善情况未呈明显量效关系。

Objective To investigate the influence of Radix Ilecis Pubescentis （RIP） on the heart function and microRNA133a （miR133a） expression in chronic heart failure （CHF） rats. Methods CHF rat model was induced by abdominal aorta coarctation for 4 weeks. Rats were divided into high-dose RIP group, low-dose RIP group, Captopril group, CHF model group and sham operatrion group. After intervention for 2 weeks, blood brain natriuretic peptide （BNP） was detected, heart function was tested by cardiac color ultrasound, and myocardial miR 133a expression was examined by fluorescence polymerase chain reaction （PCR）. Results The heart function of CHF model group was lower than the sham operation group, and the heart function was improved in the three medication groups, the difference being insignificant. The expression level of miR133a was down-regulated in CHF model group in comparison with dose RIP group e sham operation group, and was up-regulated in the in comparison with CHF model group）. Conclusion three medication groups, especially in low- CHF rats can be induced successfully by abdominal aorta coarctation, miR133a expression in the CHF rats declines when compared to the sham operation group. High- and low-dose Radix Ilecis Pubescentis can improve the heart function of CHF rats and increase miR133a expression, and the up-regulation and heart function improvement are not dose-dependent.