GC法测定克痒敏醑中薄荷脑、冰片和水杨酸甲酯的含量

Determination of Menthol,Borneol and Methyl Salicylate in Keyang Minxu Tincture by GC

目的建立同步测定克痒敏醑中薄荷脑、冰片(龙脑和异龙脑)和水杨酸甲酯含量的气相色谱(GC)方法。方法采用GC法。色谱柱为DB-WAX毛细管柱(30.0 m×0.25 mm,0.25μm);FID检测器;采用程序升温:初始温度为70℃,以7℃·min-1升至180℃,保留8 min。结果薄荷脑的加样回收率为99.9%,RSD为0.98%(n=6);、冰片(龙脑和异龙脑)加样回收率为100.8%,RSD为1.05%(n=6);水杨酸甲酯加样回收率为100.9%,RSD为1.39%(n=6)。结论本方法简便准确、重现性好、专属性强,可用于克痒敏醑的质量控制。

Objective To establish a method for the simultaneous determination of menthol, borneol （borneol and isoborneol） and methyl salicylate in Keyang Minxu tincture. Methods The gas chromatography（GC） was equipped with FID detector,and a DB-WAX（30.0 m × 0.25 mm, 0.25 μm）eapillary column was used. The temperature program of column oven was as follows： 70 ℃, increased at a rate of 7 ℃min^-1 to 180 ℃, held for 8 min. Results The average recovery of menthol, borneol （borneol and isoborneol） and methyl salieylate were 99.9 % （RSD=0.98 % ）, 100.8 %（RSD=l.05 %）, 100.9 %（RSD=l.39 %）, respectively. Conclusion The method is simple, accurate and specific. It can be used for the quality control of Keyang Minxu tincture.