2型糖尿病伴牙周病患者牙周干预后龈下菌斑中牙周致病菌的比较分析

Quantitative detection of periodontal pathogens in periodontitis patients with type 2 diabetes following basal periodontal treatment

目的观察2型糖尿病伴牙周病患者牙周干预后临床指标的变化和龈下菌斑中牙龈卟啉单胞菌(Pg)的检出量变化,初步探讨龈下微生物的检出量与牙周干预疗效之间的关系。方法根据随机数字表将确诊为2型糖尿病并伴有牙周病的患者分为实验组和对照组,记录牙周指数。实验组进行牙周干预并取龈下菌斑检测,对照组仅取龈下菌斑进行检测。6个月后复查。观察临床指标和牙龈卟啉单胞菌检出量的变化。结果基线时两组的临床指标和Pg检出量比较差异均无统计学意义(P均>0.05),6个月时,实验组临床指标值较对照组显著降低(P<0.01),Pg检出量降低,但与对照组相比差异无统计学意义(P>0.05)。经过牙周干预治疗后,实验组出血指数(SBI)和附着丧失(AL)值均减小,SBI和AL值变化差异均具有统计学意义(P<0.05);而对照组基线和6个月的临床指标值变化差异均无统计学意义。两组Pg的PCR定量检测结果在基线和6个月时变化差异均无统计学意义(P>0.05)。结论牙周干预治疗对改善牙周指数有良好效果,但是难以彻底清除牙周致病菌。

Objective To detect the changes of the levels of porphromonas gingivalis （ Pg） and clinical index and to investigate the relationship between the bacterial load and the periodontal status .Methods Periodontitis pa-tients with type 2 diabetes were randomly divided into experimental group and control group .Clinical index were ex-amined and subgingival plaques were collected before and 6 months after basal periodontal treatment in experimental group;Clinical index were examined and subgingival plaques were collected before and 6 months without any treat-ment in control group .Results Sulcus bleeding index （ SBI） and attachment loss （ AL） were significantly decreased in experimental group after 6 months （ P 〈0.01 ） , but SBI and AL were no significantly differences before and 6 months in control group （P〈0.05 ） .The numbers of Pg were no significantly differences before and 6 months in both two groups.Conclusion There were good curative effect of basal periodontal treatment in improving clinical index , but it is difficult to remove periodontal pathogens thoroughly .