用纸片扩散法和微量稀释法进行药物敏感性测定的比较

Comparative evaluation of the disk diffusion with the microdilution assay for testing the drug sensitivity

目的探讨纸片扩散法和微量稀释法用于临床常见葡萄球菌和肠杆菌的药物敏感性测定的相关性和一致性,为抗菌药物的选用提供参考依据。方法收集2011年3月至2012年2月临床分离的97株葡萄球菌和86株肠杆菌,用纸片扩散法与微量稀释法对以上临床分离的菌株进行药物敏感性测定,分析两种方法的差异。结果纸片扩散法和微量稀释法对葡萄球菌和肠杆菌药物敏感性测定结果比较,差异均无统计学意义(P<0.05);但个别药物敏感性测定结果不在可接受范围内。结论纸片扩散法和微量稀释法用于微生物药物敏感性测定的一致性较好,应注意个别药物敏感性测定结果不在可接受范围内。

Objective To compare and analyze drug sensitivity results of the disk diffusion （K-B） method and the microdilusion （MIC） method in detection of clinically common staphylococcus and enterobacteria, and provide the basis and references for the clin- ical use of antibiotics. Methods 97 staphylococcus isolates and 86 enterobacterial isolates were collected from May 2011 to Februar- y：2012：：Drug senitivity of 7 clinically commonly used antibiotics against G^＋ bacteria antibiotics and 8 antibiotics against G- bacteria were performed using both K B and MIC methods according to the CLSI standard. Data were analyzed using WHONET 5.6 soft- ware. Results The results from K-B method are highly consistent with those from the MIC method both on staphylococcus drugs and 8 enterobacteria drugs. Conclusion The K-B method and MIC method had high consistence in detecting the drug sensitivity. However,attention still needs to be paid to a few difference between them.