期刊文献+

拟南芥PHOT2基因超表达载体的构建及转化 被引量:1

下载PDF
导出
摘要 采用PCR技术扩增拟南芥PHOT2基因编码序列,并将其转入pSUPER1300超表达载体中,构建了pSUPER1300-PHOT2超表达载体,利用农杆菌介导花序转化法,转入拟南芥野生型gl1、phot1突变体中。PCR扩增及RT-PCR分析表明,该基因超表达,筛选到了潜在的转基因株系。
出处 《江苏农业科学》 北大核心 2014年第1期30-32,共3页 Jiangsu Agricultural Sciences
  • 相关文献

参考文献9

  • 1Christie J M. Phototropin blue-light receptors[J].{H}Annual Review of Plant Biology,2007.21-45.
  • 2Inoue S,Kinoshita T,Matsumoto M. Blue light-induced auto-phosphorylation of phototropin is a primary step for signaling[J].Pro-ceedings of the National Academy of Sciences of the United States of America,2008,(14):5626-5631.
  • 3Kong S G,Kinoshita T,Shimazaki K. The C-terminal kinase fragment of Arabidopsis phototropin 2 triggers constitutive phototropin responses[J].{H}Plant Journal,2007,(05):862-873.
  • 4Kong S G,Suetsugu N,Kikuchi S. Both phototropin 1 and 2 localize on the chloroplast outer membrane with distinct localization activity[J].Plant &Cell Physiology,2013,(01):80-92.
  • 5Kong S G,Suzuki T,Tamura K. Blue light-induced association of phototropin 2 with the Golgi apparatus[J].{H}Plant Journal,2006,(06):994-1005.
  • 6Takemiya A,Inoue S,Doi M. Phototropins promote plant growth in response to blue light in low light environments[J].{H}PLANT CELL,2005,(04):1120-1127.
  • 7Sakai T,Kagawa T,Kasahara M. Arabidopsis nph1 and npl1:blue light receptors that mediate both phototropism and chloroplast relocation[J].{H}Proceedings of the National Academy of Sciences(USA),2001,(12):6969-6974.
  • 8Kagawa T,Sakai T,Suetsugu N. Arabidopsis NPL1:a pho-totropin homolog controlling the chloroplast high-light avoidance response[J].{H}SCIENCE,2001,(5511):2138-2141.
  • 9Clough S J,Bent A F. Floral Dip:a simplified method for Agrobacteri-um-mediated transformation of Arabidopsis thaliana[J].Plant Jour-nal,1998,(06):735-743.

同被引文献2

引证文献1

二级引证文献1

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部