摘要
研究旨在建立一种检测饲料中AC4含量的高效液相色谱方法。饲料经乙酸乙酯提取，正己烷液液萃取净化，以甲醇-0．2％磷酸水溶液（体积比65：35）为流动相，在C18色谱柱上，紫外检测器（波长251nm）下分离检测。该方法有很好的线性关系，在添加浓度范围内，AC4的平均回收率为98．4％-106．4％，日内变异系数为0．7％～4．6％，日间变异系数为3．6％～4．3%经验证该方法操作简单，灵敏度高，准确性和重现性良好，可用于测定饲料中AC4含量的测定。
This study aimed to establish a kind of HPLC method for detecting AC4 in feed. Extraction used ethyl acetate and was cleaned up with n-hexane, and AC4 was separated on C18 column with UV detector at 251 nm, using methanol and 0.2% orthnphosphorie acid buffer （V： V=65：35） as mobile phase. This method has a good linear relationship. The average reeovery of AC4 ranged from 98.4%-106.4%, with RSD of 0.7%-4.6% within group, and 3.6%-4.3% between group. The methnd was test-ed to be of convenient, high sensitivity, good accuracy and precision.
出处
《饲料工业》
北大核心
2014年第3期54-57,共4页
Feed Industry
基金
公益性行业(农业)科研专项[201303038-1]
国家高技术研究发展计划(863计划)[2011AA10A214]
