维吾尔族子宫颈鳞癌患者血浆中DAPK基因启动子甲基化分析

Analysis of the promoter methylation of DAPK gene in plasma of Uigur cervical squamous cell carcinoma

目的:探讨新疆维吾尔族妇女子宫颈鳞癌血浆中死亡相关蛋白激酶(DAPK)基因启动子甲基化水平。方法:使用血浆游离DNA提取试剂盒提取血浆DNA,用甲基化修饰试剂盒修饰DNA,使用甲基化特异性PCR法检测血浆中DAPK基因启动子甲基化水平。结果:44例维吾尔族妇女子宫颈鳞癌患者中,有18例DAPK基因启动子甲基化,27例未发现DAPK基因启动子甲基化,甲基化率为40%(18/45);40例健康对照者检测出DAPK基因启动子甲基化阳性2例,DAPK基因启动子甲基化阴性38例,甲基化率为5%(2/40)。结论:血浆中DAPK基因启动子甲基化对诊断维吾尔族妇女子宫颈鳞癌有潜在的价值。

Objective： To explore the promoter methylation of DAPK gene level in plasma of cervical squamous cell carcinoma patients in Xinjiang Uigur, in order to prevent and treat cervical tumor. Methods： Firstly, plasma DNA was extracted with plasma free DNA extraction kit, then DNA methylation modification with methylation modification kit. Methylation specific PCR was performed to detect the promoter of DAPK gene methylation. Results： It found that there were 11 Uigur cervical squamous cell carcinoma methylated in plasma of the promoter DAPK gene and 32 cervical cancers did not find methylated in plasma of the promoter of DAPK gene. 40 normal controls did not find the promoter of DAPK gene methylation in plasma. Therefore, it had a potential value to diagnosis Uigur cervical tumor by analyzing the promoter of DAPK gene methylation in plasma. Conclusion： Examination of the promoter methylation of DAPK gene has potential value to diagnosis of cervical squamous cell carcinoma.