血清白细胞介素水平与儿童孤独症关系的研究

Association study between serum level of interleukin and autism

目的:探讨儿童孤独症与免疫系统的关系,为儿童孤独症病因学研究提供依据。方法:选取天津市4所启智学校及1所私立孤独症早教干预培训机构的孤独症儿童进行CABS、CARS、ABC量表的评定,运用酶联免疫吸附法检测血清IL-1β、IL-2R、IL-6、IL-8的水平,单因素分析血清白细胞介素与儿童孤独症的关系,相关分析孤独症儿童血清白细胞介素水平与行为特征的关系。结果:孤独症儿童血清IL-1β、IL-2R、IL-6、IL-8水平与正常参考值的差异有统计学意义(P<0.01),且高于正常参考值。孤独症儿童血清IL-1β、IL-2R、IL-6、IL-8水平与ABC量表中躯体运动项目得分呈正相关(P<0.05),与CARS量表得分相关性检验差异无统计学意义(P均>0.05)。结论:孤独症儿童可能存在免疫系统损伤,血清白细胞介素水平可能与孤独症儿童的躯体运动障碍有关。

Objective： To discuss the relationship between child autism and the immune system through the scales and biochemical experiment and provide the basis for etiology study of child autism. Methods： The autistic children from four special education schools and a private child autism intervention training institution in Tianjin were selected. Used CABS, CARS, ABC to assess, the quantification of the IL- 1β, IL -2R, IL -6, IL- 8 in the serum were determined by ELISA. Correlation between interleukin and autism, interleukin and characteristics in autism were analyzed. Results： Serum level of IL -1β, IL -2R, IL -6, IL -8 in autism were higher than those of the normal reference value （t =3. 132, P =0. 004; t = 11. 507, P =0. 000; t =9. 126, P =0. 000; t =7. 864, P =0. 000） . There were association between the serum level of IL - 1β, IL - 2R, IL - 6, IL - 8 and body movement in ABC （r = 0. 446, P = 0. 033 ; r = 0. 423, P = 0. 045 ; r = 0. 483, P = 0. 020 ; r = 0. 475, P = 0. 022） . Conclusion ： Autistic children may be immune damage, and serum interleukin level is associated with behavior disorder of children with autism.