鱼鳞肽的分离纯化及活性分析

The purification and activities analysis of fish scales peptides

利用DA201-C大孔吸附树脂对鱼鳞肽进行分离纯化,并对其活性进行分析研究。通过改变洗脱剂中乙醇的体积分数(20%、40%、60%、80%)得到不同极性的鱼鳞肽组分。随着乙醇洗脱浓度的增加,各洗脱组分灰分含量从1.20%降到0.68%,疏水性氨基酸从33.83%增加到37.67%,平均疏水值从4.25 kJ/mol增加到4.92 kJ/mol。鱼鳞肽的抗氧化活性、还原能力以及血管紧张素转化酶(angiotensin-I converting enzyme,ACE)抑制活性都随鱼鳞肽浓度的增加而增强。鱼鳞肽对DPPH·的清除作用与肽的疏水性的大小呈反比关系,而对超氧阴离子自由基(O2-·)的清除作用、还原能力以及对ACE活性的抑制作用与肽的疏水性呈正相关。与抗氧化活性和还原能力相比,相同浓度下鱼鳞肽具有更高的ACE抑制活性,且鱼鳞肽的活性与其极性密切相关。

DA201-C macro-porous absorption resins were used to purify the fish scales peptides.Then the activities of the fish scales peptides were analyzed.The different elution fractions with different polarity peptides were obtained by changing the alcohol concentration.The ash content of fractions decreased from 1.20% to 0.68% with increased ethanol concentrations,while the content of hydrophobic amino acids and the average hydrophobicity of each eluted fraction increased from 33.83% to 37.67% and from 4.25 kJ/mol to 4.92 kJ/mol respectively.The antioxidant activity,reducing capacity and angiotensin-I converting enzyme（ ACE） inhibitory ability increased with peptides concentrations increase.The DPPH· scavenging effect of fish scale peptides decreased when the alcohol concentration increased,while the O 2-· scavenging effect,reducing capacity and ACE inhibitory ability increased.Compared to the antioxidant activity and reducing capacity,the ACE inhibitory ability of the fish scales peptides was stronger at the same concentration.The activities of the fish scales peptides was related with its polarity.