摘要
目的:优选土木香中总酚酸和绿原酸的提取工艺。方法:以总酚酸和绿原酸提取量为指标,在单因素试验基础上,通过正交试验考察提取时间、乙醇体积分数、固液比及提取温度对微波提取工艺的影响。采用UV测定总酚酸含量;HPLC测定绿原酸含量,流动相乙腈-0.4%磷酸溶液(13∶87),检测波长327 nm。结果:最佳微波提取工艺条件为35%乙醇,提取时间5 min,固液比1∶23,提取温度75℃;总酚酸、绿原酸提取量分别为(6.78±0.19),(1.53±0.09)mg·g-1。结论:优选的微波提取工艺具有效率高、能耗低等优点,适用于工业化生产。
Objective: To optimize extraction technology of total phenolic acids and chlorogenic acid from Inulae Radix. Method: With extracting amounts of total phenolic acids and chlorogenic acid as indexes, based on single factor tests, orthogonal test was adopted to optimize microwave-assisted extraction technology by taking extraction time, ethanol concentration, solid-liquid ratio and extraction temperature as factors. The content of total phenolic acids was determined by UV; HPLC was adopted to determine the content of chlorogenic acid with mobile phase of acetonitrile-0.4% phosphoric acid (13: 87) and detection wavelength at 327 nm. Result: Optimum extraction conditions were as follows: ethanol concentration of 35% ; extracted 5 minutes with solid- liquid ratio of 1 : 23, extraction temperature at 75 ℃ ; Under these conditions, extracting amounts of total phenolic acids and chlorogenic acid were (6.78 ± 0. 19) and ( 1.53 ± 0.09) mg . g-1, respectively. Conclusion: Optimized microwave-assisted extraction process was effective with low energy consumption, which was suitable for industrial production.
出处
《中国实验方剂学杂志》
CAS
北大核心
2014年第4期22-24,共3页
Chinese Journal of Experimental Traditional Medical Formulae
基金
河北省中医药管理局项目(2010046)
河北北方学院课题(Q2013029)
关键词
土木香
微波提取工艺
正交设计
单因素试验
总酚酸
绿原酸
Inulae Radix
microwave-assisted extraction process
orthogonal design
total phenolicacids
chlorogenic acid