土木香中总酚酸和绿原酸的微波辅助提取工艺优选

Optimization of Microwave-assisted Extraction Process for Total Phenolic Acids and Chlorogenic Acid from Inulae Radix

目的:优选土木香中总酚酸和绿原酸的提取工艺。方法:以总酚酸和绿原酸提取量为指标,在单因素试验基础上,通过正交试验考察提取时间、乙醇体积分数、固液比及提取温度对微波提取工艺的影响。采用UV测定总酚酸含量;HPLC测定绿原酸含量,流动相乙腈-0.4%磷酸溶液(13∶87),检测波长327 nm。结果:最佳微波提取工艺条件为35%乙醇,提取时间5 min,固液比1∶23,提取温度75℃;总酚酸、绿原酸提取量分别为(6.78±0.19),(1.53±0.09)mg·g-1。结论:优选的微波提取工艺具有效率高、能耗低等优点,适用于工业化生产。

Objective： To optimize extraction technology of total phenolic acids and chlorogenic acid from Inulae Radix. Method： With extracting amounts of total phenolic acids and chlorogenic acid as indexes, based on single factor tests, orthogonal test was adopted to optimize microwave-assisted extraction technology by taking extraction time, ethanol concentration, solid-liquid ratio and extraction temperature as factors. The content of total phenolic acids was determined by UV; HPLC was adopted to determine the content of chlorogenic acid with mobile phase of acetonitrile-0.4% phosphoric acid （13： 87） and detection wavelength at 327 nm. Result： Optimum extraction conditions were as follows： ethanol concentration of 35% ; extracted 5 minutes with solid- liquid ratio of 1 ： 23, extraction temperature at 75 ℃ ; Under these conditions, extracting amounts of total phenolic acids and chlorogenic acid were （6.78 ± 0. 19） and （ 1.53 ± 0.09） mg . g-1, respectively. Conclusion： Optimized microwave-assisted extraction process was effective with low energy consumption, which was suitable for industrial production.