核桃仁的定性定量鉴别方法

Identification of TLC and Determination of HPLC on Juglandis Semen

目的：建立核桃仁TLC鉴别和HPLC含量测定方法。方法：采用TLC法对核桃仁中glansregininsA进行定性鉴别并采用HPLC法对其进行含量测定。DiamonsilC18色谱柱（4．6mm×200mm，5μm），流速0．8mL·min-1，检测波长265nm，流动相乙腈-1％冰醋酸，梯度洗脱。结果：在TLC鉴别中，glansregininsA斑点清晰、分离效果好；在HPLC测定中，glansregininsA在24．26-242．6mg·L-1线性关系良好，平均回收率为96．4％（RSD2．7％）。结论：所建立的TLC和HPLC法操作简便、专属性强且重复性好，可作为核桃仁定性、定量的质量控制方法。

Objective： To establish the methods of identification of TLC and determination of HPLC in the Juglandis Semen. Method： Glansreginins A in Juglandis Semen was identified by TLC method and the content was determined by HPLC method. The chromatographic column was Diamonsil Cis （4.6mm×200mm,5μm）. The flow rate was 0.8 mL .min-1, the detection wavelength was set at 265 nm. A gradient elution of acetonitrile- water containing 1% acetic acid was used. Result： The TLC method had high sensitivity and showed clear spots of glansreginins A; the glansreginins A showed a good linearity within the range of 24.26-242.6 mg .L-1, and the average recovery rate was 96.4% （RSD 2.7% ）. Conclusion： The methods are simple, reliable and repeatable. They can be used for the quality control of Juglandis Semen.