竹叶柴胡根中皂苷类成分的薄层鉴别及含量测定

TLC Identification and Content Determination of Saponins from the Roots of Bupleurum marginatum

目的：研究竹叶柴胡根中的皂苷类成分，为其人药提供科学依据。方法：采用薄层色谱法对皂苷类成分进行定性鉴别，并采用高效液相色谱法首次对竹叶柴胡根中皂苷类成分进行含量测定，用C18（4．60mm×150mm，5μm）柱，以乙腈一水为流动相梯度洗脱，检测波长210nm，流速0．8mL·min-1，柱温35℃，进样量10μL。结果：竹叶柴胡根中含有皂苷类成分。柴胡皂苷a和柴胡皂苷d的线性范围分别为440-8800ng（r=0．9997），488—9760ng（r=0．9992）线性关系良好，平均加样回收率（n=6）分别为99．88％（RSD0．41％），100．23％（RSD0．46％）。样品中柴胡皂苷a和柴胡皂苷d的总量在0．48％-1．08％。结论：竹叶柴胡全草人药具有其科学性。

Objective： Studies on the saponins from the roots of Bupleurum marginatum. Method： The thin layer chromatography （TLC） was performed to identify saponins qualitatively, and a HPLC method was used for the determination of saponins in the roots of B. marginatum. The RP-HPLC system consisting of C18 （4.60 mm × 150 mm, 5μm） column and a solution system of acetonitrile-water with gradient elution, the detection wavelength was at 210 nm, the flow rate was 0.8 mL .min-1 , the column temperature was set at 35 ℃and the injection volume was 10 μL. Result： The linear ranges of saikosaponin A and saikosaponin D were 440-8 800 ng （r =0. 999 7） , 488-9 760 ng （r =0. 999 2） respectively. The average recoveries （n =6） were 99.88% （RSD 0.41% ） , 100.23% （RSD 0.46% ） respectively. The amounts of saikosaponin A and saikosaponin D were in the range of 0.48% -1.08%. Conclusion： The whole grass of B. marginatum as a traditional chinese medicine has its scientific nature.