藜芦酸在大鼠体内的药代动力学

Pharmacokinetics of Veratric Acid in Rats

目的：考察藜芦酸在大鼠体内的药代动力学。方法：大鼠口服灌胃给予藜芦酸，在不同时间点采集血样，选择3，4-二羟基苯甲酸甲酯为内标，采用HPLC测定血药浓度，流动相乙腈（A）-1％乙酸（B）梯度洗脱（0-11min，5％-57％A；11～13min，57％～5％A），检测波长256nm，绘制血药浓度-时间曲线，通过Kinetica4．4软件计算药代动力学参数。结果：灌胃给药后藜芦酸在大鼠体内的达峰时间分别为（45．00±10．00），（31．00±8．22）min，存在二次达峰现象；高、低剂量组的Cmax分别为（176．85±28．89），（68．14±12．58）mg·L-1，t1/2分别为（87．74±20．74），（227．18±93．78）min，AUC0-∞分别为（50363．50±11667．7），（18830．32±4354．35）min·mg·L-1，MRT0-∞分别为（201．39±16．72），（227．40±21．37）min。结论：藜芦酸口服吸收较快，消除较慢，体内作用时间较长。

Objective： To study pharmacokinetics of veratric acid in rats. Method： Veratric acid was administrate to rats by oral gavage, blood samples were collected at various time points, HPLC was adopted to determine the content of veratric acid in blood samples by taking 3, 4-dihydroxybenzoic acid methyl ester as an internal standard, mobile phase of acetonitrile （A） -1% acetic acid （B） （0-11 min, 5% -57% A ; 11-13 min, 57%-5% A ）, detection wavelength was set at 256 nm, plasma concentration-time curve was drawn, pharmacokinetic parameters were calculated by Kinetica 4.4 software. Result： After oral gavage administration, veratric acid existed secondary peak phenmenon in rats, Tmax were （45.00 ±10.00） and （31.00 ± 8.22） min, respectively; Cmax of high and low dose groups were （ 176.85 ±28.89） and （68.14 ± 12.58） mg .L-1 , t1/2 were （87.74±20.74） and （227.18 ±93.78） min, AUC0-∞ were （50 363.50 ±11 667.7） and （18 830.32 ± 4354.35） min .mg -L-1, MRT0-∞ were （201.39± 16.72） and （227.40± 21.37） min, respectively. Conclusion： Veratrie acid had a long in vivo acting time due to its quick absorption and slow elimination.