芍药甘草汤对角叉菜胶诱导的炎症相关指标时序性变化研究

Study on Time-effect Relationships of Shaoyao Gancao Decoction on Carrageenan-induced Inflammatory Markers

目的：研究中药复方芍药甘草汤对角叉菜胶诱导肿胀及相关炎症指标的时序变化关系。方法：SD大鼠随机分为正常组、模型组、芍药甘草汤高（4．8g·kg-1）、低（2．4g·kg-1）剂量组。各给药组单次给药，给药30min后皮下注射入-角叉菜胶建立大鼠足跖肿胀急性炎症模型，观察芍药甘草汤在造模后1，2，3，4，5，6，7，8，9，10，11，12h不同时间点对大鼠足跖肿胀率、髓过氧化物酶（MPO）、环氧化酶-2（COX-2）、前列腺素E2（PGE2）炎症指标的影响，并研究其时效变化关系。结果：足跖皮下注射角叉菜胶后，模型组足跖肿胀明显，与模型组比较，芍药甘草汤高低剂量均能明显抑制角叉菜胶致大鼠的足肿胀度，高剂量在1～6h各时间点对足跖肿胀均有明显抑制作用（P〈0．05，P〈0．01），低剂量组在2，4，5，6h对足跖肿胀有明显抑制作用（P〈0．05，P〈0．01）；与正常组比较，模型组MPO在1-6h、COX-2在2-8h，11h、PGE2在1-6，8-12h明显升高（P〈0．05，P〈0．01）；与模型组比较，芍药甘草汤在2，4，5，6h对MPO的含量有明显抑制作用，在4，5，6h对COX-2的含量有明显抑制作用，在2,3，5，6h对PGE2的含量有明显抑制作用。芍药甘草汤在2，6h对MPO和PGE2的浓度变化均有显著抑制作用，但仅在6h对COX-2的浓度变化有明显抑制作用。结论：中药复方芍药甘草汤对角叉菜胶诱导的不同炎症指标肿胀率有明显降低作用，浓度不同作用时间也不尽相同，对MPO，COX-2及PEG2均具有明显降低作用，但各指标起效时间不同；具有明显的时序变化特点。

Objective： To investigate the time-effect relationship of Shaoyao Gancao decoction on carrageenan-induced inflammatory markers. Method： Acute inflammatory model was induced by carrageenane. Time-effect relation of paw swelling inhibition ratio, myeloperoxidase （MPO）, cyclooxygenase-2 （COX-2）, prostaglandinE2 （PGE2） was tested respectively atl, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12h. Result： The result showed that Shaoyao Gancao decoction had significant efficacy of anti-inflammatory. Inflammatory markers such as paw swelling inhibition ratio, MPO, COX-2, PGE2 were inhibited at different time points obviously. High- dose Shaoyao Gancao decoction could effectively reduce carrageenan-induced paw edema at 1, 2, 3, 4, 5, 6 h.It also could significantly decrease the production of MPO at 2, 4, 5, 6 h, COX-2 at 4, 5, 6 h, PGE2 at 2, 3, 5, 6 h. But low-does Shaoyao Ganeao decoction could effectively reduce carrageenan-induced paw edema at 2, 4, 5, 6 h, and decrease the production of MPO, PGE2 at 2, 6 h, and COX-2 only at 6 h. Conclusion： Shaoyao Gancao decoction had significantly inhibit effects on carrageenan-induced different inflammatory markers （swelling rate, MPO, COX-2 and PEG2 ） in the timing relationship at different some time, and different concentration Shaoyao Gancao decoction had different action time. It shows that Shaoyao Gancao decoction have the characteristic of the time-effect relationship.