益母草注射液主要提取成分对凝血系统影响的筛选

Main Ingredients from Motherwort Injection for Coagulation System

目的:探索益母草注射液中主要提取成分对凝血系统的影响。方法:通过体外实验测定凝血四项值、血小板聚集率和纤溶活性探索益母草注射液主要成分对凝血系统的影响。结果:体外实验表明,葫芦巴碱具有明显延长活化部分凝血酶时间(APTT)的作用,氯化胆碱和葫芦巴碱均具有抗血小板聚集的作用,均无明显的纤溶活性。结论:氯化胆碱和葫芦巴碱为益母草注射液抗凝的有效成分。有效成分的提取分离为扩大益母草注射液的临床应用以及控制益母草注射液的质量提供了很好的科学依据。

Objective： To explore the main ingredients from Motherwort injection for coagulation system. Method： By measuring the coagulation values, platelet aggregation and fibrinolysis to explore the effects of the main ingredients from Motherwort injection on blood coagulation system. Result： In vitro, trigonelline obviously prolonged activated partial thromboplastin time （APTT） , both choline chloride and trigonelline inhibited platelet aggregation, but no significant fibrinolytic activity. Conclusion： Both choline chloride and trigonelline are the active ingredients of Motherwort injection for coagulation system. The extraction and separation of active ingredients expand the clinical application of Motherwort injection and provide a good scientific basis for the quality control of Motherwort injection.