调脂通络胶囊对高脂模型心肌缺血再灌注大鼠氧自由基和抗氧化酶的影响

Effect of Tiaozhi Tongluo Capsule on Oxygen Free Radicals and Antioxidant Enzymes of Myocardial Ischemia Reperfusion Injury

目的:测量心肌缺血再灌注损伤(myocardial ischemic reperfusion injury,MIRI)后大鼠血清中的血脂、心肌酶和心肌匀浆中的丙二醛(malondialdehyde,MDA)和相关抗氧化酶的含量。观察调脂通络胶囊可能的心肌保护作用和相关的机制。方法:本实验在高脂血症模型的基础上建立MIRI模型,将56只大鼠分为调脂通络胶囊高、中、低剂量组(8.64,4.32,2.16 g·kg-1·d-1)、高脂模型组、非高脂模型组、阿托伐他汀阳性对照组和高脂假手术组,每组8只大鼠。喂食4周高脂饲料(非高脂模型组正常饮食)后药物干预或生理盐水灌胃4周,结扎左冠状动脉前降支30 min(假手术组只穿线不结扎),松线再灌注24 h后采血测定血清血脂(TC,TG,HDL,LDL)含量,血清心肌酶(LDH,CK-MB)活性;取缺血区心肌组织测定大鼠心肌匀浆中抗氧化酶[超氧化物歧化酶(SOD),谷胱甘肽过氧化物酶(GSH-Px),过氧化氢酶(CAT)]活性,MDA的含量。结果:与正常饮食组相比,高脂模型组的血脂、心肌酶、MDA明显升高,抗氧化酶含量明显降低;各药物组均可以明显降低心肌酶和MDA含量、升高抗氧化酶SOD,CAT,GSH-Px的活性,调脂通络胶囊各剂量组与他汀组无统计学差异。结论:调脂通络胶囊和阿托伐他汀具有减少自由基聚集,提高抗氧化酶活力的作用。

Objective： To observe the myocardial protective effect and related mechanism of the Tiaozhi Tongluo capsule （TZTL）. Method： MIRI model combined with hyperlipemia was established, 56 male Wistar rats were divided into following seven group： hyperlipemia group, normal group, sham group without hyperlipemia, Atorva statin group, TZTL 8.64, 4.32, 2. 16 g. kg-1.d-1 group. After feeding of high fat diet for 4 weeks, the drug intervention was carried out, then the left arteria coronariia was legated for 30 min, following reperfusion for 24 hours. At the end of the experiment, the blood and myocardial homogenate samples were collected for measurement of malondialdehyde （MDA）, superoxide dismutase （SOD）, catalase （CAT） and glutathione peroxidase （GSH-Px） for blood lipids, cardiac enzymes [ lactic clehydrogense （LDH） , creatine kinase isoenzyme （CK-MB）]. Result： The blood lipids,, cardiac enzymes and MDA levels were significantly increased and antioxidant enzyme content decreased obviously in model group. All treatment groups could decrease ihe cardiac enzymes and MDA levels, and increase the SOD, CAT and GSH-Px levels. Conclusion： The TZTL can reduce the free radicals agrregation and improve the effectof antioxidase.