土茯苓对急性汞中毒大鼠的保护作用研究

Role of Smilax glabra in Preventing Acute Mercury Toxicity in Rats

目的:探讨土茯苓对急性汞中毒大鼠的保护作用。方法:将大鼠随机分为正常对照组、模型组、土茯苓高、中、低剂量组(5,10,20 g·kg-1),模型组及土茯苓高、中、低剂量组sc 2.5 mg·kg-1HgCl2溶液,连续2 d,造成汞中毒,正常对照组sc生理盐水;土茯苓水煎液低、中、高剂量组末次染毒后开始ig给药,ig体积10 mL·kg-1,2次/d,早晚各1次,持续3 d,观察土茯苓对染汞大鼠血清尿素氮(BUN)、汞、尿蛋白、尿乳酸脱氢酶(LDH)、尿碱性磷酸酶(ALP)活力、尿汞、肾汞、肝汞含量及肾脏病理组织学变化的影响。结果:与正常对照组比较,模型组尿汞、血汞和肝汞、肾皮质汞含量,尿LDH及ALP活力,尿蛋白和血清BUN含量均显著提高(P<0.01),肾脏损伤明显;与模型组对比,土茯苓水煎液高、中剂量组血清BUN、尿蛋白,尿LDH及ALP活力,肾、肝及血汞含量显著降低,尿汞含量显著增加(P<0.01或P<0.05);肾脏病理组织学观察结果显示,高剂量组土茯苓对汞中毒大鼠肾损伤有较好的修复作用。结论:土茯苓具有能够改善汞中毒大鼠肝肾功能、去除体内汞蓄积的作用,对汞中毒大鼠具有一定的防治作用。

Objective： To investigate the role of Smilax glabra in the protective effect of acute mercurypoisoning in rats. Method： The rats were divided into five groups： normal control group, model group, high, middle and low dose of S. glabra group. The control group was only given 0.9% saline by subcut aneousinjection andthe other groupswere injected with HgC12of 2.5 mg .kg-1 once a day for two days, the high, middle and low dose of S. glabra group were orally given twice a day for three days after the last injection, the influence of S. glabra aqueous extract on the serum blood urea nitrogen （BUN）, lactate dehydrogenase （LDH） and alkaline phosphatase （ALP） , the urinary protein, the levels of mercury in urine, renal cortex and liver and the renal histopathological changeswereobserved. Result： Compared with the blank control group, the serum BUN, the urinary activities of LDH and ALP, the urinary protein, and the levels of mercury in urine, renal cortex and liverfrom the chlorinated mercury groupwere increased significant （P 〈 0.01 ）. Further more, compared with the model group, the serum BUN, the urinary activities of LDH and ALP, the urinary protein, and the levels of mercury in urine, renal cortex and liver from the high or medium S. glabra aqueous extract group were reduced significant （P 〈 0. 01or P 〈 0.05）. The results of kidney pathological histology observation showedc that S. glabra had an obviously improvementon the acute mercury poisoningrats. Conclusion： S. glabra Can improve the liver and kidney function of mercury poisoning in rats and decrease the accumulation of mercury in vivo, the S. glabra shows definite preventive and therapeutic effect on acute mercury poisoned rats.